Nanotechnology and Integrated Bio-Engineering Center, School of Engineering, University of Ulster, Jordanstown, Co Antrim, Northern Ireland, UK.
J Hazard Mater. 2011 Jul 15;191(1-3):56-61. doi: 10.1016/j.jhazmat.2011.04.035. Epub 2011 Apr 16.
The aim of this study is to assess in vitro cytotoxic effects of titania nanostructures and carbon nanotubes (CNTs) by exposing A549 lung epithelial cell line to these materials. Titania nanotubes (TiNTs) were grown by hydrothermal treatment of TiO(2) nanoparticles, followed by annealing them at 400°C. The titania nanostructures obtained on annealing (mixture of nanotubes and nanorods) were hollow and open ended, containing 3-5 layers of titania sheets, with an internal diameter ∼3-5 nm and external diameter ∼8-10 nm, and a specific surface area of 265 m(2)/g. As-supplied single walled (SWCNTs) and microwave plasma enhanced chemical vapour deposition (MPCVD) grown multi walled carbon nanotubes (MWCNTs) were used in this study. The lengths and diameters of the SWCNTs were 5-10nm and 0.5-3 nm respectively. The lengths and diameters of the MWCNTs were 25-30 μm and 10-30 nm respectively. The cell viability was evaluated using the MTT (3-(4,-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium) assay. No significant cytotoxic effects of titania nanostructures were observed over a period of a week of testing time, while the presence of CNTs in some cases demonstrated significant cytotoxic effects. Finally, possible reason of cytotoxicity is discussed in the light of microstructures of materials.
本研究旨在通过将 A549 肺上皮细胞系暴露于这些材料来评估二氧化钛纳米结构和碳纳米管 (CNT) 的体外细胞毒性效应。通过水热处理 TiO2 纳米颗粒，然后在 400°C 下退火来生长二氧化钛纳米管 (TiNTs)。退火得到的二氧化钛纳米结构（纳米管和纳米棒的混合物）是空心和开口的，包含 3-5 层二氧化钛片，内径约为 3-5nm，外径约为 8-10nm，比表面积为 265m2/g。本研究中使用了供应的单壁碳纳米管 (SWCNT) 和微波等离子体增强化学气相沉积 (MPCVD) 生长的多壁碳纳米管 (MWCNT)。SWCNT 的长度和直径分别为 5-10nm 和 0.5-3nm。MWCNT 的长度和直径分别为 25-30μm 和 10-30nm。通过 MTT（3-(4,-二甲基噻唑-2-基)-2,5-二苯基四唑）测定评估细胞活力。在一周的测试时间内，没有观察到二氧化钛纳米结构的明显细胞毒性效应，而在某些情况下 CNT 的存在表现出明显的细胞毒性效应。最后，根据材料的微观结构讨论了细胞毒性的可能原因。
