鉴定新型含有苏氨酸、天冬氨酸和谷氨酸作为碱基部分的哺乳动物磷脂。

Identification of novel mammalian phospholipids containing threonine, aspartate, and glutamate as the base moiety.

机构信息

Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Nov 1;879(29):3296-302. doi: 10.1016/j.jchromb.2011.04.033. Epub 2011 May 6.

DOI:10.1016/j.jchromb.2011.04.033

PMID:21601537

Abstract

In this study, we showed the occurrence of phosphatidyl-L-threonine (PThr), phosphatidyl-L-aspartate (PAsp), and phosphatidyl-L-glutamate (PGlu) in rat brain. Analyses using an HPLC-ESI-MS and an amino acid analyzer showed the presence of L-threonine, L-aspartate, and L-glutamate in the acid-hydrolysates of phospholipids from porcine cerebrum, rat cerebrum, and rat liver. Results of ESI-MS/MS analyses with neutral loss scanning and product ion scanning suggest the presence of PThr-(18:0, 18:1), PThr-(18:0, 22:6), PAsp-(18:0, 18:1), PAsp-(18:0, 22:6), PGlu-(18:0, 18:1), and PGlu-(18:0, 22:6) in rat brain. This is the first study to identify 2 novel phospholipids, PAsp and PGlu, with a carboxylate-phosphate anhydride bond, in living organisms.

摘要

在这项研究中，我们展示了磷脂酰-L-苏氨酸（PThr）、磷脂酰-L-天冬氨酸（PAsp）和磷脂酰-L-谷氨酸（PGlu）在大鼠脑中的存在。使用 HPLC-ESI-MS 和氨基酸分析仪的分析表明，猪脑、鼠脑和鼠肝磷脂酸水解产物中存在 L-苏氨酸、L-天冬氨酸和 L-谷氨酸。中性丢失扫描和产物离子扫描的 ESI-MS/MS 分析结果表明，大鼠脑中存在 PThr-（18:0,18:1）、PThr-（18:0,22:6）、PAsp-（18:0,18:1）、PAsp-（18:0,22:6）、PGlu-（18:0,18:1）和 PGlu-（18:0,22:6）。这是首次在活生物体中鉴定出具有羧酸盐-磷酸酐键的 2 种新型磷脂，PAsp 和 PGlu。