University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Gründensrasse 40, CH-4132 Muttenz, Switzerland.
Environ Pollut. 2011 Oct;159(10):3086-96. doi: 10.1016/j.envpol.2011.04.013. Epub 2011 May 24.
Residues of the UV-filter 2-ethyl-hexyl-4-trimethoxycinnamate (EHMC) are ubiquitously found in aquatic biota but potential adverse effects in fish are fairly unknown. To identify molecular effects and modes of action of EHMC we applied a gene expression profiling in zebrafish using whole genome microarrays. Transcriptome analysis and validation of targeted genes were performed after 14 days of exposure of male zebrafish. Concentrations of 2.2 μg/L and 890 μg/L EHMC lead to alteration of 1096 and 1137 transcripts, respectively, belonging to many pathways. Genes involved in lipid metabolism and estrogenic pathway (vtg1), lipid biosynthesis (ptgds), vitamin A metabolic process (rbp2a), DNA damage and apoptosis (gadd45b), and regulation of cell growth (igfbp1a) were investigated by qRT-PCR analysis in whole body, liver, brain and testis. The analysis showed tissue-specific gene profiles and revealed that EHMC slightly affects the transcription of genes involved in hormonal pathways including vtg1, esr1, esr2b, ar, cyp19b and hsd17β3.
EHMC（2-乙基己基-4-三甲氧基肉桂酸酯）的紫外线滤光剂残留普遍存在于水生生物群中，但鱼类的潜在不良影响尚不清楚。为了确定 EHMC 的分子效应和作用模式，我们使用全基因组微阵列在斑马鱼中进行了基因表达谱分析。在雄性斑马鱼暴露 14 天后，进行了转录组分析和靶向基因的验证。EHMC 的浓度为 2.2μg/L 和 890μg/L 时，分别导致 1096 和 1137 个转录本发生改变，这些转录本属于许多途径。通过 qRT-PCR 分析，在整体、肝脏、大脑和睾丸中研究了参与脂质代谢和雌激素途径（vtg1）、脂质生物合成（ptgds）、维生素 A 代谢过程（rbp2a）、DNA 损伤和细胞凋亡（gadd45b）以及细胞生长调节（igfbp1a）的基因。分析显示了组织特异性基因图谱，并表明 EHMC 对包括 vtg1、esr1、esr2b、ar、cyp19b 和 hsd17β3 在内的激素途径相关基因的转录有轻微影响。
