University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Gründenstrasse 40, 4132 Muttenz, Switzerland; University of Basel, Division of Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, Klingelbergstrasse 50, 4056 Basel, Switzerland.
University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Gründenstrasse 40, 4132 Muttenz, Switzerland.
Sci Total Environ. 2014 Apr 1;476-477:207-17. doi: 10.1016/j.scitotenv.2014.01.015. Epub 2014 Jan 24.
Wide application of the UV-filter octocrylene (OC) in cosmetics leads to contamination of the aquatic environment, but effects of OC remain unclear. Here we determine bioaccumulation and molecular effects of OC. Adult male zebrafish were exposed to 22, 209 and 383 μg/L and embryos to 69, 293 and 925 μg/L OC. OC accumulated in fish up to 17 μg/g. Calculated BCF varied between 41 and 136. Microarray analysis in brain and liver following exposure to 383 μg/L OC revealed alteration of 628 and 136 transcripts, respectively. Most prominent GO processes included developmental processes, organ development, hematopoiesis, formation of blood vessels, blood circulation, fat cell differentiation and metabolism. Validation by RT-qPCR in brain and liver of adult fish and embryos included a series of genes. Blood levels of 11-ketotestosterone were not altered. The transcriptomics data suggest that OC mainly affects transcription of genes related to developmental processes in the brain and liver as well as metabolic processes in the liver.
UV 滤光剂辛氧烷（OC）在化妆品中的广泛应用导致了水环境污染，但 OC 的影响尚不清楚。在这里，我们确定了 OC 的生物积累和分子效应。雄性成年斑马鱼暴露于 22、209 和 383μg/L，胚胎暴露于 69、293 和 925μg/L 的 OC。OC 在鱼类中积累高达 17μg/g。计算的 BCF 介于 41 和 136 之间。暴露于 383μg/L OC 后，大脑和肝脏的微阵列分析显示分别有 628 和 136 个转录本发生改变。最显著的 GO 过程包括发育过程、器官发育、造血、血管形成、血液循环、脂肪细胞分化和代谢。在成年鱼和胚胎的大脑和肝脏中通过 RT-qPCR 进行验证包括一系列基因。11-酮睾酮的血液水平没有改变。转录组学数据表明，OC 主要影响大脑和肝脏中与发育过程以及肝脏中代谢过程相关的基因的转录。
