Medical Biology Research Center, Medical School, Kermanshah University of Medical Sciences, Daneshgah Avenue, Kermanshah, Iran.
Angiology. 2012 Feb;63(2):131-7. doi: 10.1177/0003319711409741. Epub 2011 May 20.
There are conflicting reports about the association of endothelial nitric oxide synthase (eNOS) gene polymorphism and the risk of coronary artery disease (CAD). To determine the frequency of eNOS G894T variant and to find the possible association between this polymorphism with CAD we studied 207 unrelated patients with total CAD (with and without diabetes) and 92 controls. The eNOS variants were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The presence of GT + TT genotype was associated with 2.1-fold (P = .006), 2.29-fold (P = .006), and 1.93-fold (P = .032) increased risk of CAD in total CAD, CAD with diabetes, and in CAD without diabetes patients, respectively. The presence of T allele of eNOS increased the risk of CAD 2.15-fold (P = .001). The levels of low-density lipoprotein cholesterol (LDL-C) and triglycerides (TG) tended to be higher in patients carrier for T allele compared to those with G allele. The results of present study revealed that eNOS G894T polymorphism is associated with increased risk of CAD in our population.
关于内皮型一氧化氮合酶（eNOS）基因多态性与冠心病（CAD）风险的关联，目前存在相互矛盾的报告。为了确定 eNOS G894T 变体的频率，并发现该多态性与 CAD 之间可能的关联，我们研究了 207 名无关联的总 CAD（伴或不伴糖尿病）患者和 92 名对照者。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测 eNOS 变体。GT + TT 基因型的存在与 CAD 总发生率、合并糖尿病的 CAD 发生率和无糖尿病的 CAD 发生率分别增加 2.1 倍（P =.006）、2.29 倍（P =.006）和 1.93 倍（P =.032）。eNOS 的 T 等位基因的存在使 CAD 的风险增加了 2.15 倍（P =.001）。与携带 G 等位基因的患者相比，携带 T 等位基因的患者的低密度脂蛋白胆固醇（LDL-C）和甘油三酯（TG）水平往往更高。本研究结果表明，eNOS G894T 多态性与我们人群中 CAD 风险增加相关。
