Jia Haitao, Tao Hong, Feng Ruopeng, Li Meiyu, Bai Jie, Sun Tao, Wen Jinhua, Hu Qikuan
Physiological Department, Cranial Cerebral Disease Lab, Ningxia Medical University, Ningxia, People's Republic of China.
Neuroreport. 2011 Jun 22;22(9):448-52. doi: 10.1097/WNR.0b013e3283476b46.
Neural stem/progenitor cells transit from fibroblast growth factor-responsive to epidermal growth factor (EGF)-responsive stem cells in the subventricular zone (SVZ). Here, we provide evidences that Pax6 plays a crucial role in the regulation of the EGF-responsive stem cell pool of the SVZ. Using Pax6 homozygous mutant mice (E18.5d), we found that the neurospheres were formed less than that from the wild-type mice, and the expression of EGF receptor in these neurospheres is downregulated very much. The amount of EGF-responsive cells in the mutant dorsal cortex SVZ (E18.5d) was also decreased from 16.8 (wild) to 4.5% (mutant), by flow cytometry method. Immunostaining of the cortex showed a downregulation of EGF receptor expression. All these results suggest that Pax6 regulate the EGF-responsive stem cells in the SVZ.
神经干细胞/祖细胞在脑室下区（SVZ）从对成纤维细胞生长因子有反应的干细胞转变为对表皮生长因子（EGF）有反应的干细胞。在此，我们提供证据表明Pax6在调控SVZ中对EGF有反应的干细胞池方面发挥着关键作用。利用Pax6纯合突变小鼠（胚胎第18.5天），我们发现其形成的神经球比野生型小鼠少，并且这些神经球中EGF受体的表达大幅下调。通过流式细胞术方法检测，突变型背侧皮质SVZ（胚胎第18.5天）中对EGF有反应的细胞数量也从16.8%（野生型）降至4.5%（突变型）。皮质的免疫染色显示EGF受体表达下调。所有这些结果表明Pax6调控SVZ中对EGF有反应的干细胞。
