Arumugam N D, Ajam N, Blackall P J, Asiah N M, Ramlan M, Maria J, Yuslan S, Thong K L
Microbiology Division, Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Trop Biomed. 2011 Apr;28(1):55-63.
One hundred and fourteen strains of Pasteurella multocida were isolated from different domestic animals species (cattle, buffalo, sheep, goat, pig, rabbit, dog, cat), avian species (chicken, duck, turkey) and wild animals (deer, tiger, orang utan, marmoset). The serogroups of P. multocida were determined by both conventional capsular serotyping and a multiplex PCR assay targeting specific capsular genes. Based on the conventional serotyping method, the 114 strains of P. multocida were subtyped into 55 species-specific (untypeable strains) P. multocida, 15 serogroup A, 23 serogroup B and 21 serogroup D. Based on the multiplex PCR assay on the specific capsular genes associated with each serogroup, the 114 strains were further divided to 22 species-specific P. multocida (KMT1 - 460 bp), 53 serogroup A (A - 1,044 bp), 33 serogroup B (B - 760 bp) and 6 serogroup D (D - 657 bp). No serogroup E (511 bp) or F (851 bp) was detected among the Malaysian P. multocida. PCR-based typing was more discriminative and could further subtype the previously untypeable strains. Overall, there was a significant and positive correlation between both methods in serogrouping P. multocida (r = 0.7935; p<0.4893). Various serogroups of P. multocida were present among the livestock with 75% of the strains belonging to serogroups A or B. PCR serotyping was therefore a highly species-specific, sensitive and robust method for detection and differentiation of P. multocida serogroups compared to conventional serotyping. To the best of our knowledge, this is the first report from Malaysia of the application of a PCR to rapidly define the species-specific P. multocida and its serogroups as an important zoonotic pathogen in Malaysia.
从不同家畜物种（牛、水牛、绵羊、山羊、猪、兔、狗、猫）、禽类物种（鸡、鸭、火鸡）和野生动物（鹿、老虎、猩猩、狨猴）中分离出114株多杀性巴氏杆菌。通过传统的荚膜血清分型和靶向特定荚膜基因的多重PCR检测来确定多杀性巴氏杆菌的血清群。基于传统血清分型方法，114株多杀性巴氏杆菌被分为55株物种特异性（不可分型菌株）多杀性巴氏杆菌、15株血清群A、23株血清群B和21株血清群D。基于对与每个血清群相关的特定荚膜基因的多重PCR检测，114株菌株进一步分为22株物种特异性多杀性巴氏杆菌（KMT1 - 460 bp）、53株血清群A（A - 1,044 bp）、33株血清群B（B - 760 bp）和6株血清群D（D - 657 bp）。在马来西亚的多杀性巴氏杆菌中未检测到血清群E（511 bp）或F（851 bp）。基于PCR的分型更具鉴别力，能够进一步对先前不可分型的菌株进行亚型分类。总体而言，两种方法在多杀性巴氏杆菌血清群分类方面存在显著正相关（r = 0.7935；p<0.4893）。家畜中存在各种多杀性巴氏杆菌血清群，75%的菌株属于血清群A或B。因此，与传统血清分型相比，PCR血清分型是一种高度物种特异性、灵敏且稳健的检测和区分多杀性巴氏杆菌血清群的方法。据我们所知，这是马来西亚首次报道应用PCR快速确定作为马来西亚重要人畜共患病原体的物种特异性多杀性巴氏杆菌及其血清群。
