Dini L, Mattioli P
Department of Biology, University of Tor Vergata Rome, Italy.
Biosci Rep. 1990 Feb;10(1):37-46. doi: 10.1007/BF01116849.
We studied the carbohydrate recognition systems on liver sinusoidal cells of adult chicken and 20-day-old embryos. We localized and quantified the binding sites for glycoproteins exposing terminal N-acetylglucosamine (GlcNAc), mannose and galactose (Gal) residues. Sinusoidal liver cells from animals of both ages express on their cell surfaces binding sites for GlcNAc, mannose and galactose residues, while hepatocytes bind glycoproteins with GlcNAc residues. The gold particles distribution on Kupffer cells depend on the binding sites and the age considered. Binding sites for GlcNAc and Gal residues are generally present as clusters of gold granules, while mannose-specific binding sites are always as single gold granules. Ligand-gold complexes bound on endothelial cells are always present on the coated regions of the cell surface. The number of GlcNAc and Gal-specific receptors expressed on the cell surface of Kupffer cells undergoes modifications between embryonal and adult life.
我们研究了成年鸡和20日龄胚胎肝脏窦状细胞上的碳水化合物识别系统。我们对暴露末端N - 乙酰葡糖胺（GlcNAc）、甘露糖和半乳糖（Gal）残基的糖蛋白结合位点进行了定位和定量。两个年龄段动物的肝脏窦状细胞在其细胞表面均表达GlcNAc、甘露糖和半乳糖残基的结合位点，而肝细胞则结合含有GlcNAc残基的糖蛋白。库普弗细胞上金颗粒的分布取决于结合位点和所考虑的年龄。GlcNAc和Gal残基的结合位点通常以金颗粒簇的形式存在，而甘露糖特异性结合位点总是单个金颗粒。结合在内皮细胞上的配体 - 金复合物总是存在于细胞表面的被覆区域。库普弗细胞表面表达的GlcNAc和Gal特异性受体的数量在胚胎期和成年期之间会发生变化。
