Chakera A H, Nielsen B S, Madsen J, Romer J, Kristjansen P E G, Buch I, Binderup T, Ingvar C, Nalla A, Kjaer A, Hesse B
Clinic of Plastic Surgery, Breast Surgery and Burns Unit, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark.
Int J Mol Imaging. 2011;2011:828151. doi: 10.1155/2011/828151. Epub 2011 Apr 28.
Aim. To develop a method and obtain proof-of-principle for immunolymphoscintigraphy for identification of metastatic sentinel nodes. Methods. We selected one of four tumour-specific antibodies against human breast cancer and investigated (1), in immune-deficient (nude) mice with xenograft human breast cancer expressing the antigen if specific binding of the intratumorally injected, radioactively labelled, monoclonal antibody could be scintigraphically visualized, and (2) transportation to and retention in regional lymph nodes of the radioactively labelled antibody after subcutaneous injection in healthy rabbits. Results and Conclusion. Our paper suggests the theoretical possibility of a model of dual isotope immuno-lymphoscintigraphy for noninvasive, preoperative, malignant sentinel node imaging.
目的。开发一种用于识别转移性前哨淋巴结的免疫淋巴闪烁成像方法并获得原理验证。方法。我们从四种抗人乳腺癌的肿瘤特异性抗体中选择一种，并进行研究：（1）在表达该抗原的人乳腺癌异种移植免疫缺陷（裸）小鼠中，观察经瘤内注射放射性标记的单克隆抗体后，其特异性结合是否能通过闪烁成像观察到；（2）在健康兔皮下注射放射性标记抗体后，观察其向区域淋巴结的转运和滞留情况。结果与结论。我们的论文提出了一种双同位素免疫淋巴闪烁成像模型用于无创、术前恶性前哨淋巴结成像的理论可能性。
