Korea Institute of Ceramic Engineering & Technology, Seoul 153-801, Republic of Korea.
Chem Commun (Camb). 2011 Sep 28;47(36):9989-91. doi: 10.1039/c1cc11664a. Epub 2011 May 23.
An enzyme-magnetic nanoparticle conjugate is prepared via conjugation of Ni(2+) ions onto the surface of magnetic nanoparticles to interact with a six histidine-tagged enzyme. The catalytic properties and enzyme rigidification of the conjugates are more stable at high concentrations of aromatic hydrocarbons.
通过将 Ni(2+) 离子共轭到磁性纳米粒子的表面上来制备酶-磁性纳米粒子缀合物,以与具有六个组氨酸标签的酶相互作用。在芳香烃浓度较高的情况下,缀合物的催化性质和酶刚性更稳定。