使用硫醇功能化磁性纳米颗粒对混合蛋白质进行快速和选择性分离。
Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles.
作者信息
Lee Soo Youn, Ahn Chi Young, Lee Jiho, Lee Jin Hyung, Chang Jeong Ho
机构信息
Bio-IT Convergence Center, Korea Institute of Ceramic Engineering and Technology, Seoul, 153-801, South Korea.
出版信息
Nanoscale Res Lett. 2012 May 31;7(1):279. doi: 10.1186/1556-276X-7-279.
Thiol group functionalized silica-coated magnetic nanoparticles (Si-MNPs@SH) were synthesized for rapid and selective magnetic field-based separation of mixed proteins. The highest adsorption efficiencies of binary proteins, bovine serum albumin (BSA; 66 kDa; pI = 4.65) and lysozyme (LYZ; 14.3 kDa; pI = 11) were shown at the pH values corresponding to their own pI in the single-component protein. In the mixed protein, however, the adsorption performance of BSA and LYZ by Si-MNPs@SH was governed not only by pH but also by the molecular weight of each protein in the mixed protein.
合成了巯基官能化的二氧化硅包覆磁性纳米粒子（Si-MNPs@SH），用于基于磁场快速、选择性地分离混合蛋白质。在单一组分蛋白质中，二元蛋白质牛血清白蛋白（BSA；66 kDa；pI = 4.65）和溶菌酶（LYZ；14.3 kDa；pI = 11）在对应于各自pI的pH值下表现出最高吸附效率。然而，在混合蛋白质中，Si-MNPs@SH对BSA和LYZ的吸附性能不仅受pH值影响，还受混合蛋白质中各蛋白质分子量的影响。
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