大鼠支持细胞中组织型纤溶酶原激活物信使核糖核酸水平的调节，以及睾丸管周细胞中纤溶酶原激活物抑制剂信使核糖核酸水平的调节。

Modulation of levels of messenger RNA for tissue-type plasminogen activator in rat Sertoli cells, and levels of messenger RNA for plasminogen activator inhibitor in testis peritubular cells.

作者信息

Nargolwalla C, McCabe D, Fritz I B

机构信息

Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.

出版信息

Mol Cell Endocrinol. 1990 Mar 26;70(1):73-80. doi: 10.1016/0303-7207(90)90060-l.

DOI:10.1016/0303-7207(90)90060-l

PMID:2160384

Abstract

Messenger RNA for tissue-type plasminogen activator has been detected in RNA extracts from rat Sertoli cells in culture. Relative levels are increased in Sertoli cells stimulated by follicle-stimulating hormone or by dibutyryl cyclic AMP (dbcAMP) and decreased in cells maintained in the presence of transforming growth factor beta, type 1 (TGF-beta 1). Messenger RNA for plasminogen activator inhibitor, type 1 (PAI-1) has been detected in RNA extracts from rat peritubular myoid cells. Relative levels are increased in peritubular cells stimulated by TGF-beta 1, and decreased by the presence of dbcAMP in the medium. Data are interpreted to indicate that net protease activities in the seminiferous tubule are regulated at transcriptional levels by endocrine and paracrine agents.

摘要

在培养的大鼠支持细胞的RNA提取物中已检测到组织型纤溶酶原激活物的信使核糖核酸。在促卵泡激素或二丁酰环磷腺苷（dbcAMP）刺激的支持细胞中，其相对水平升高；而在转化生长因子β1（TGF-β1）存在的情况下维持培养的细胞中，其相对水平降低。在大鼠睾丸肌样周细胞的RNA提取物中已检测到1型纤溶酶原激活物抑制剂（PAI-1）的信使核糖核酸。在TGF-β1刺激的睾丸周细胞中，其相对水平升高，而培养基中dbcAMP的存在则使其降低。这些数据被解释为表明生精小管中的净蛋白酶活性受内分泌和旁分泌因子在转录水平上的调节。