Memon Mushtaq A, Anway Matthew D, Covert Trevor R, Uzumcu Mehmet, Skinner Michael K
Department of Veterinary Clinical Sciences, Washington State University, Pullman, WA 99164-4234, USA.
Mol Cell Endocrinol. 2008 Nov 6;294(1-2):70-80. doi: 10.1016/j.mce.2008.08.017. Epub 2008 Aug 26.
The role transforming growth factor beta (TGFb) isoforms TGFb1, TGFb2 and TGFb3 have in the regulation of embryonic gonadal development was investigated with the use of null-mutant (i.e. knockout) mice for each of the TGFb isoforms. Late embryonic gonadal development was investigated because homozygote TGFb null-mutant mice generally die around birth, with some embryonic loss as well. In the testis, the TGFb1 null-mutant mice had a decrease in the number of germ cells at birth, postnatal day 0 (P0). In the testis, the TGFb2 null-mutant mice had a decrease in the number of seminiferous cords at embryonic day 15 (E15). In the ovary, the TGFb2 null-mutant mice had an increase in the number of germ cells at P0. TGFb isoforms appear to have a role in gonadal development, but interactions between the isoforms is speculated to compensate in the different TGFb isoform null-mutant mice.
利用针对转化生长因子β（TGFβ）各亚型（即TGFβ1、TGFβ2和TGFβ3）的无效突变（即基因敲除）小鼠，研究了TGFβ亚型在胚胎性腺发育调控中的作用。研究的是胚胎后期性腺发育，因为纯合子TGFβ无效突变小鼠通常在出生前后死亡，胚胎期也会有一些死亡。在睾丸中，TGFβ1无效突变小鼠出生时（出生后第0天，P0）生殖细胞数量减少。在睾丸中，TGFβ2无效突变小鼠在胚胎第15天（E15）时生精索数量减少。在卵巢中，TGFβ2无效突变小鼠在P0时生殖细胞数量增加。TGFβ亚型似乎在性腺发育中起作用，但推测不同TGFβ亚型无效突变小鼠中各亚型之间的相互作用起到了补偿作用。
