Institute of Animal Science, Chinese Academy of Agricultural Sciences, 2-Yuan-Ming-Yuan West Rd., Haidian District, Beijing 100193, People's Republic of China.
Mol Biol Rep. 2012 Feb;39(2):1349-57. doi: 10.1007/s11033-011-0869-0. Epub 2011 May 21.
Ethylene responsive factor (ERF) proteins play important roles in plant growth and development and regulate biotic and abiotic stress responses. In this study, a full length mRNA encoding a novel ERF-type transcription factor namely Ceratoides arborescens ERF protein (CeERF) was isolated from C. arborescens. The deduced amino acid of CeERF had a conserved APETALA2/ERF (AP2/ERF) domain which specifically binds to cis-acting elements GCC box. Under normal conditions, the expression level of CeERF was highest in leaves and lowest in roots. CeERF expression was induced by 20% PEG in a time-dependent pattern and peaked at 8 h. CeERF also acts in salt- and hormones-induced stresses. Transient expression analysis in onion epidermal cells indicated that CeERF protein localized to nucleus. Overexpression of CeERF in transgenic tobacco plants resulted in higher tolerance to abiotic stresses than in control plants. These results suggested that CeERF might play a role in abiotic stress signal transduction and that overexpression of CeERF might serve as a feasible approach to enhance resistance in forage, even crop.
乙烯响应因子（ERF）蛋白在植物生长发育中发挥重要作用，并调节生物和非生物胁迫反应。在本研究中，从驼绒藜中分离到一个全长编码一个新的 ERF 型转录因子的 mRNA，即 Ceratoides arborescens ERF 蛋白（CeERF）。CeERF 的推导氨基酸具有保守的 APETALA2/ERF（AP2/ERF）结构域，该结构域特异性结合顺式作用元件 GCC 盒。在正常条件下，CeERF 的表达水平在叶片中最高，在根中最低。CeERF 的表达受 20%PEG 的诱导，呈时间依赖性模式，在 8 小时达到峰值。CeERF 还参与盐和激素诱导的胁迫。洋葱表皮细胞中的瞬时表达分析表明，CeERF 蛋白定位于细胞核。在转基因烟草植物中过表达 CeERF 导致对非生物胁迫的耐受性高于对照植物。这些结果表明 CeERF 可能在非生物胁迫信号转导中发挥作用，过表达 CeERF 可能是增强饲料，甚至作物抗性的一种可行方法。
