Hemadi M, Saki G, Shokri S, Ghasemi F M
Physiology Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Folia Morphol (Warsz). 2011 Feb;70(1):18-23.
This study evaluates the effect of melatonin on follicular dynamics in neonate vitrified ovarian grafts. Vitrified ovaries from neonate F1 hybrid mice, candidates for transplantation to treated or non-treated groups, were thawed under standard conditions with or without the addition of 100 μM melatonin, respectively. Following transplantation, melatonin (20 mg/kg/day) or saline solution was injected i.p. into the treated and the non-treated groups, respectively. Vaginal cytology to monitor estrogenic activity together with follicle survival and development in the ovary grafts was examined. The results showed that the sites of transplantation became obvious within the oestrous phase. Histological analysis showed that there was a dynamic of the ovogenesis process in the vitrified ovary grafts. However, in the ovary graft the empty cavity together with jumbled oocyte-granulosa complex in the non-treated group was higher than in the melatonin treated group. Overall, the number of primary follicles in the ovary grafts of both groups remained constant throughout the oestrous period. However, the treated ovary grafts contained more secondary follicles throughout the oestrous period in comparison to non-treated ovary grafts. The antral follicle rates were more marked in the melatonin treated group than in the non-treated group during the oestrous period, whereas at other days of the cycle no significant difference was observed. The fresh corpora lutea rates were significantly higher in the treatment group than in the non-treatment group. The study showed that there is a positive effect with melatonin resulting in more grafts restoring puberty. Furthermore, the associated increase in healthy follicles suggests that melatonin has a preventative ischaemia/antioxidant action and may be useful to follicles.
本研究评估褪黑素对新生小鼠玻璃化卵巢移植中卵泡动态的影响。将来自新生F1杂交小鼠、准备移植到治疗组或未治疗组的玻璃化卵巢，分别在标准条件下解冻，解冻时一组添加100 μM褪黑素，另一组不添加。移植后，分别向治疗组和未治疗组腹腔注射褪黑素（20 mg/kg/天）或生理盐水。通过阴道细胞学检查监测雌激素活性，并检查卵巢移植中的卵泡存活和发育情况。结果显示，移植部位在发情期内变得明显。组织学分析表明，玻璃化卵巢移植中有卵泡发生过程的动态变化。然而，未治疗组卵巢移植中的空卵泡腔以及混乱的卵母细胞-颗粒细胞复合体比褪黑素治疗组更多。总体而言，两组卵巢移植中的初级卵泡数量在整个发情期保持恒定。然而，与未治疗的卵巢移植相比，治疗组的卵巢移植在整个发情期含有更多的次级卵泡。在发情期，褪黑素治疗组的窦状卵泡率比未治疗组更明显，而在周期的其他日子未观察到显著差异。治疗组的新鲜黄体率显著高于未治疗组。该研究表明，褪黑素具有积极作用，可使更多移植恢复青春期。此外，健康卵泡数量的相关增加表明褪黑素具有预防缺血/抗氧化作用，可能对卵泡有益。
