Sapmaz Ekrem, Ayar Ahmet, Celik Husnu, Sapmaz Tansel, Kilic Nermin, Yasar M Akif
Department of Obstetrics and Gynecology, Faculty of Medicine, Firat University, Elazig-Turkey.
Neuro Endocrinol Lett. 2003 Oct;24(5):350-4.
To comparatively investigate the effects melatonin and oxytetracycline as antioxidants on autologous intraperitoneal ovary transplantation in rats.
28 adult female Wistar rats with regular estrous cycles were randomly divided into four groups. Group 1 (n=7) was the control group; Group 2 (n=7) bilateral oophorectomy and intraperitoneal transplantation group; and Groups 3 (n=7) and 4 (n=7) firstly received 20 mg/kg/IP melatonin and 10 mg/kg/IP oxytetracycline., respectively and fifteen minutes after that, they had bilateral oophorectomy and intraperitoneal transplantation. Ovarian tissue and blood malondialdehyde (MDA) levels, superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) levels were determined. Vaginal cytology and ovarian necrosis were histopathologically investigated. Kruskall Wallis analysis of variance was used in the statistical analysis of data.
Ovarian necrosis, tissue MDA and plasma MDA levels were significantly higher in Group 2 compared to the Groups 1, 3 and 4 (p<0.03). SOD and GSHPx levels were significantly lower in group 2 compared to the groups 1, 3 and 4 (p<0.03). Melatonin was found to be more effective on ovarian necrosis and tissue MDA level than the use of oxytetracycline following autologous intraperitoneal ovary transplantation (p<0.03). There was a significant positive correlation between tissue MDA level and ovarian necrosis and a very strong negative correlation between tissue MDA and SOD level (rs = +/-0.9, n=28, p=0.000).
Although both melatonin and oxytetracycline were found to be effective in autologous intraperitoneal pelvic ovary transplantation, melatonin is more effective against both the ovarian necrosis and tissue MDA level in rats.
比较褪黑素和土霉素作为抗氧化剂对大鼠自体腹腔内卵巢移植的影响。
28只成年雌性Wistar大鼠，发情周期规律，随机分为四组。第1组（n = 7）为对照组；第2组（n = 7）为双侧卵巢切除术及腹腔内移植组；第3组（n = 7）和第4组（n = 7）分别先腹腔注射20mg/kg褪黑素和10mg/kg土霉素，15分钟后行双侧卵巢切除术及腹腔内移植。测定卵巢组织和血液中丙二醛（MDA）水平、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSHPx）水平。对阴道细胞学和卵巢坏死进行组织病理学研究。数据统计分析采用Kruskal Wallis方差分析。
与第1、3、4组相比，第2组的卵巢坏死、组织MDA和血浆MDA水平显著更高（p < 0.03）。与第1、3、4组相比，第2组的SOD和GSHPx水平显著更低（p < 0.03）。发现褪黑素在自体腹腔内卵巢移植后对卵巢坏死和组织MDA水平的作用比土霉素更有效（p < 0.03）。组织MDA水平与卵巢坏死之间存在显著正相关，组织MDA与SOD水平之间存在非常强的负相关（rs = +/-0.9，n = 28，p = 0.000）。
虽然褪黑素和土霉素在大鼠自体腹腔内盆腔卵巢移植中均有效，但褪黑素对大鼠卵巢坏死和组织MDA水平的作用更有效。
