van Els C A, Bakker A, van Rood J J, Goulmy E
Department of Immunohematology and Blood Bank, University Hospital Leiden, The Netherlands.
Hum Immunol. 1990 May;28(1):39-50. doi: 10.1016/0198-8859(90)90101-t.
We studied the accessory cell requirements for triggering in vivo primed human major histocompatibility complex class I- and class II-restricted T cells specific for minor histocompatibility antigens. We compared the antigen-presenting capacities of peripheral blood lymphocytes (PBLs) and Epstein-Barr virus-transformed lymphoblastoid cell lines (EBV-LCLs), both derived from the same donor, to induce minor histocompatibility antigen-specific cytotoxic and proliferative T cells. PBLs and EBV-LCLs were equally effective as antigen-presenting cells to trigger cytotoxic-T-cell responses specific for minor histocompatibility antigens, some of which were reactive with B cells only. In contrast, a clear difference was found between the capacities of the two antigen-presenting cell types to induce minor histocompatibility antigen-specific T-helper-cell responses. PBLs as antigen-presenting cells could induce T-helper-cell lines reactive against minor histocompatibility antigens presented on PBLs, on EBV-LCLs, or on both cell types as stimulator cells. Unexpectedly, however, EBV-LCLs as antigen-presenting cells in all instances failed to induce T-helper-cell responses specific for minor histocompatibility antigens presented on PBLs or on both PBLs and EBV-LCLs as stimulator cells and could only trigger T-helper cells directed against B-cell-specific minor histocompatibility antigens. Our findings indicate a dichotomy in the capacity of EBV-LCLs to present minor histocompatibility antigens in the induction versus the effector phse of the in vitro T-helper-cell response. Furthermore, the results show different in vitro accessory cell requirements for major histocompatibility complex class I- and class II-restricted T-cell responses specific for human minor histocompatibility antigens.
我们研究了触发体内针对次要组织相容性抗原的、由人类主要组织相容性复合体I类和II类限制的T细胞致敏所需的辅助细胞条件。我们比较了来自同一供体的外周血淋巴细胞（PBL）和爱泼斯坦-巴尔病毒转化的淋巴母细胞系（EBV-LCL）作为抗原呈递细胞，诱导针对次要组织相容性抗原的特异性细胞毒性和增殖性T细胞的能力。PBL和EBV-LCL作为抗原呈递细胞，在触发针对次要组织相容性抗原的细胞毒性T细胞反应方面同样有效，其中一些反应仅与B细胞有关。相比之下，发现这两种抗原呈递细胞类型在诱导针对次要组织相容性抗原的T辅助细胞反应的能力上存在明显差异。作为抗原呈递细胞的PBL可以诱导针对呈递于PBL、EBV-LCL或这两种细胞类型作为刺激细胞上的次要组织相容性抗原的反应性T辅助细胞系。然而，出乎意料的是，作为抗原呈递细胞的EBV-LCL在所有情况下都未能诱导针对呈递于PBL或PBL和EBV-LCL两者作为刺激细胞上的次要组织相容性抗原的T辅助细胞反应，而只能触发针对B细胞特异性次要组织相容性抗原的T辅助细胞。我们的研究结果表明，EBV-LCL在体外T辅助细胞反应的诱导阶段与效应阶段呈递次要组织相容性抗原的能力存在二分法。此外，结果显示针对人类次要组织相容性抗原的主要组织相容性复合体I类和II类限制的T细胞反应在体外对辅助细胞的需求不同。
