Zhu Li-Guo, Chen Xin, Yu Jie, Gao Jing-Hua, Wang Shang-Quan, Feng Min-Shan, Yin He
Department of the Second Spine, Wangjing Hospital of China Academy of Traditional Chinese Medicine Sciences, Beijing 100102, China.
Zhongguo Gu Shang. 2011 Apr;24(4):327-31.
To explore the effects of removing dampness and promoting diuresis method on autoimmune and immuno-inflammatory response caused by nucleus pulposus of rats, in order to provide the basis for the treatment of lumbar disc herniation with Chinese medical immunotherapy.
Forty male Wistar rats were divided into 4 groups randomly according to body weight layer:sham operation group (group A), model contrast group (group B), colchicine tablets group (group C), modified Qingyao decoction group (group D). There were 10 rats in each group. Nucleus pulposus of coccygeal vertebra was transplanted to the gluteal muscle by operation in groups B, C, D, which can lead to autoimmune and immuno-inflammatory response of rats; the rats of group A were only treated with sham operation. At the 3rd day after operation, the rats were fed through intragastric administration, the group A and B with distilled water (10 ml/kg), the group C and D respectively with suspension of colchicine tablets (10 ml/kg, 0.01 mg/ml) and water-decocted liquid of modified Qingyao decoction (10 ml/kg,1.035 g/ml), once a day and continuous medication for 18 days. All rats were killed at the 21th day after operation. The immunoglobulin G (IgG), immunoglobulin M (IgM) and interleukin-1beta (IL-1beta), interleukin-8 (IL-8) level in serum of different groups were detected by ELISA method. At the same time, surrounding tissues of the transplanted nucleus pulposus were observed by pathological section.
The level of IgG, IgM, IL-1beta, IL-8 in serum of group B was significantly higher than that of group A (P < 0.01), while the level of IgG, IgM, IL-1beta, IL-8 in serum of group C, D was significantly lower than that of group B (P < 0.05 or P < 0.01). Moreover, pathological section indicated that immuno-inflammatory response was hardly found in surgical site of group A, while local immuno-inflammatory response of surrounding tissues of the transplanted nucleus pulposus of group C and D was much lighter than that of group B.
Removing dampness and promoting diuresis method could inhibit autoimmune and immuno-inflammatory response caused by nucleus pulposus of rats.
探讨祛湿利水法对大鼠髓核所致自身免疫及免疫炎症反应的影响，为中医免疫疗法治疗腰椎间盘突出症提供依据。
40只雄性Wistar大鼠按体重分层随机分为4组：假手术组（A组）、模型对照组（B组）、秋水仙碱片组（C组）、加味清尧汤组（D组）。每组10只。B、C、D组通过手术将尾椎髓核移植至臀肌，可引发大鼠自身免疫及免疫炎症反应；A组大鼠仅行假手术。术后第3天开始灌胃给药，A、B组给予蒸馏水（10 ml/kg），C、D组分别给予秋水仙碱片混悬液（10 ml/kg，0.01 mg/ml）和加味清尧汤水煎液（10 ml/kg，1.035 g/ml），每日1次，连续用药18天。术后第21天处死所有大鼠。采用ELISA法检测不同组大鼠血清中免疫球蛋白G（IgG）、免疫球蛋白M（IgM）及白细胞介素-1β（IL-1β）、白细胞介素-8（IL-8）水平。同时，对移植髓核周围组织进行病理切片观察。
B组血清中IgG、IgM、IL-1β、IL-8水平显著高于A组（P < 0.01），而C、D组血清中IgG、IgM、IL-1β、IL-8水平显著低于B组（P < 0.05或P < 0.01）。此外，病理切片显示A组手术部位几乎未发现免疫炎症反应，而C、D组移植髓核周围组织的局部免疫炎症反应明显轻于B组。
祛湿利水法可抑制大鼠髓核所致的自身免疫及免疫炎症反应。
