Zhao Li, Ren Jiao, Feng Jing, Pang Zheng, Zhang Zhong-Xian, Tan Wen-Jie, Tian Hou-Wen, Ruan Li
Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2010 Dec;24(6):406-8.
To identify specific T lymphocyte epitope on E6 protein of human papillomavirus type 18 in mice.
Infection with one recombinant vaccinia virus rVVJ18 E7, E6 respectively in C57 BL/6 and BALB/c mice, specific cellular immune responses were detected by ELISPOT or intracellular cytokine stainings by using a series of overlapping synthetic peptides covering full length of the amino acid sequence of E6 and E7 proteins or various truncated peptides.
The rVVJ18 E7, E6 generated significant E6 specific T-cell immune responses in vaccinated mice. Mapping of the epitope of E6 revealed that the peptides E6(67-75 ( KCIDFYSRI) and E6(60-68) (IPHAAGHKC) presented respectively by C57BL/6 and BALB/c mice were the optimal peptides to activate E6-specific CD8+ T lymphocytes. However no positive cellular immune responses stimulated with various E7 peptides were detected by ELISPOT in immunized mice.
Two specific T lymphocyte epitopes were identified on E6 protein in C57BL/6 and BALB/c mice, which will provide the basis to evaluate cellular immune response elicited by HPV18 E6 protein based vaccine.
在小鼠中鉴定人乳头瘤病毒18型E6蛋白上的特异性T淋巴细胞表位。
分别用一种重组痘苗病毒rVVJ18 E7、E6感染C57 BL/6和BALB/c小鼠，通过ELISPOT或细胞内细胞因子染色，使用一系列覆盖E6和E7蛋白氨基酸序列全长的重叠合成肽或各种截短肽来检测特异性细胞免疫反应。
rVVJ18 E7、E6在接种疫苗的小鼠中产生了显著的E6特异性T细胞免疫反应。E6表位图谱显示，C57BL/6和BALB/c小鼠分别呈递的肽E6(67 - 75 (KCIDFYSRI)和E6(60 - 68) (IPHAAGHKC)是激活E6特异性CD8 + T淋巴细胞的最佳肽。然而，在免疫小鼠中通过ELISPOT未检测到各种E7肽刺激产生的阳性细胞免疫反应。
在C57BL/6和BALB/c小鼠的E6蛋白上鉴定出两个特异性T淋巴细胞表位，这将为评估基于HPV18 E6蛋白的疫苗引发的细胞免疫反应提供依据。
