He Dian, Bai Rui-Ting, Zhang Yong, Shan Zhi-Lan, Li Li, Liu Jie, Ma Jian-Xin, Zhang Xiao-Xi, Yan Dong-Mei, Xu Wen-Bo
Chaoyang Center for Disease Control and Prevention, Beijing 100021, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2010 Dec;24(6):421-3.
To study the genetic characterizations of VP1 region of Human enterovirus 71 (HEV71) isolated from clinical specimens of hand, foot and mouth disease (HFMD) patients in Beijing in 2008.
285 clinical samples were collected from HFMD patients in hospitals and day-care centers in Chaoyang district. They were performed by reverse transcription-polymerase chain reaction (RT-PCR) specific for HEV71. 10 HEV71 isolates were selected for entire VP1 coding gene amplification and sequencing.
129 samples were RT-PCR positive, the positive rate is 45.26%. The homology of the nucleotide and the amino acid of the 10 strains were 94.6%-99.6% and 95.9%-100%. The phylogenetic tree revealed that 10 Beijing strains clustered within the C4a evolution branch of C4 subgenotype.
RT-PCR played an important role in identifying HFMD outbreak in Beijing in 2008. The HEV71 strains were all belong to C4a evolution branch of C4 subgenotype with several transmission chains, and it showed that C4 subgenotype HEV71 spread in mainland China widely after 1998. The molecular epidemiology surveillance and the research of genetic characterizations of HEV71 should be strengthened in mainland China.
研究2008年从北京手足口病（HFMD）患者临床标本中分离出的人肠道病毒71型（HEV71）VP1区的基因特征。
从朝阳区医院和日托中心的手足口病患者中收集285份临床样本。采用针对HEV71的逆转录-聚合酶链反应（RT-PCR）进行检测。选择10株HEV71分离株进行VP1编码基因全长扩增及测序。
129份样本RT-PCR阳性，阳性率为45.26%。10株病毒核苷酸和氨基酸同源性分别为94.6% - 99.6%和95.9% - 100%。系统发育树显示，10株北京分离株聚集在C4亚型的C4a进化分支内。
RT-PCR在2008年北京手足口病疫情鉴定中发挥了重要作用。HEV71毒株均属于C4亚型的C4a进化分支，存在多条传播链，表明1998年后C4亚型HEV71在中国大陆广泛传播。中国大陆应加强HEV71的分子流行病学监测和基因特征研究。
