Department of Molecular Physics, Huygens Laboratory, Leiden University, The Netherlands.
J Phys Chem A. 2011 Sep 1;115(34):9552-6. doi: 10.1021/jp112413d. Epub 2011 Jun 7.
We compare the resonance Raman spectra acquired at two excitation wavelengths, 496.5 and 514.5 nm, of spheroidene in the wild-type reaction center of Rhodobacter sphaeroides and reconstituted into the reaction center of the Rhodobacter sphaeroides mutant R26. Our earlier work showed that the reconstituted R26 reaction center holds spheroidene in two configurations: 15,15'-cis and another configuration. Here we show that in the wild-type reaction center only 15,15'-cis spheroidene is present. In the resonance Raman spectra of the reconstituted R26 reaction centers, a transition is identified that arises exclusively from the second configuration. According to density-functional-theory calculations, this transition is specific for the 13,14-cis configuration.
我们比较了在两个激发波长 496.5nm 和 514.5nm 下获取的野生型球形红细菌反应中心和重组到球形红细菌突变体 R26 反应中心中的球形多烯的共振拉曼光谱。我们之前的工作表明，重组的 R26 反应中心中的球形多烯存在两种构象：15,15'-顺式和另一种构象。在这里，我们表明在野生型反应中心中仅存在 15,15'-顺式球形多烯。在重组的 R26 反应中心的共振拉曼光谱中，确定了一个仅由第二种构象产生的跃迁。根据密度泛函理论计算，该跃迁是 13,14-顺式构象所特有的。
