Kok P, Köhler J, Groenen E J, Gebhard R, van der Hoef I, Lugtenburg J, Hoff A F, Farhoosh R, Frank H A
Centre for the Study of Excited States of Molecules, Huygens Laboratory, Leiden University, The Netherlands.
Biochim Biophys Acta. 1994 Apr 28;1185(2):188-92. doi: 10.1016/0005-2728(94)90209-7.
We report resonance Raman spectra of the carotenoid spheroidene and its 14'-13C and 15'-13C substituted analogues in petroleum ether and bound to the reaction centre of Rhodobacter sphaeroides R26. The spectra in petroleum ether correspond to planar all-trans spheroidene while those of the reaction centres are consistent with a nonplanar 15,15'-cis spheroidene. The effect of 13C labelling is largest in the carbon-carbon double-bond stretching region. The 15'-13C substitution of the reaction centre bound spheroidene, however, hardly changes the C=C band as compared to that for the natural abundance spheroidene apart from a new weak band at 1508 cm(-1). This observation has been interpreted as a decoupling of the C15=C15' stretch from the other double-bond stretches in combination with a small intrinsic Raman intensity of this local mode for 15,15'-cis spheroidene.
我们报告了类胡萝卜素球形烯及其14'-13C和15'-13C取代类似物在石油醚中以及与球形红细菌R26反应中心结合时的共振拉曼光谱。石油醚中的光谱对应于平面全反式球形烯，而反应中心的光谱与非平面15,15'-顺式球形烯一致。13C标记的影响在碳 - 碳双键伸缩区域最为显著。然而，与天然丰度的球形烯相比，反应中心结合的球形烯的15'-13C取代除了在1508 cm(-1)处出现一个新的弱带外，几乎不改变C = C带。这一观察结果被解释为C15 = C15'伸缩与其他双键伸缩解耦，同时15,15'-顺式球形烯的这种局域模式的拉曼本征强度较小。
