Janero D R, Burghardt C
Roche Research Center, Department of Pharmacology and Chemotherapy, Hoffmann-La Roche Inc., Nutley, NJ 07110.
J Chromatogr. 1990 Mar 16;526(1):11-24. doi: 10.1016/s0378-4347(00)82479-2.
Solid-phase extraction methods using pre-packed silica cartridges and various elution solvents have been developed and evaluated as chromatographic means to enrich biological lipid extracts for platelet-activating factor (PAF). The optimized procedure advanced selectively removed the major tissue/blood neutral lipids and non-choline-containing phospholipids from complex lipid mixtures and yielded thereby a choline phospholipid fraction markedly enriched in bioactive PAF. Some tested solid-phase extraction procedures, while capable of resolving choline phospholipids from other polar and non-polar species, were detrimental to PAF's bioactivity and evidenced considerable loss or degradation of this analyte. It is concluded that, with solvents of appropriate composition, strength and polarity, solid-phase extraction on silica cartridges has several unique advantages over conventional thin-layer and column chromatographic methods presently in use for PAF enrichment from biological sources.
已开发并评估了使用预填充硅胶柱和各种洗脱溶剂的固相萃取方法，作为从生物脂质提取物中富集血小板活化因子（PAF）的色谱手段。优化后的方法能从复杂脂质混合物中选择性地去除主要的组织/血液中性脂质和不含胆碱的磷脂，从而得到显著富集生物活性PAF的胆碱磷脂馏分。一些经过测试的固相萃取方法虽然能够从其他极性和非极性物质中分离出胆碱磷脂，但对PAF的生物活性有害，且该分析物有相当大的损失或降解。结论是，使用组成、强度和极性合适的溶剂，硅胶柱上的固相萃取相对于目前用于从生物来源富集PAF的传统薄层和柱色谱方法具有几个独特的优势。
