Department of Molecular Oncology and Imaging, Institute of Cancer, Queen Mary University of London, London, UK.
Acta Oncol. 2011 Jun;50 Suppl 1:53-5. doi: 10.3109/0284186X.2010.525224.
The collection of tissue from the prostate gland for research creates unique challenges in the identification of cancer and in preserving pathological material.
Formalin fixed paraffin embedded (FFPE) tissue is often available in abundance after pathological processing and reporting of specimens but is limited in value for detailed molecular tests. Tissue micro-array if carefully performed is a helpful technique for examining many FFPE specimens with immunohistochemical or fluorescence in situ hybridization tests.
The collection of fresh tissue prior to formalin fixation and later validation samples of fresh prostate cancer is difficult as prostate cancer is very difficult to identify macroscopically on cut prostate specimens. Also, the act of manipulation and dissection of the gland while fresh and without compromising surgical margins is challenging. Methods which have been used to dissect the fresh prostate gland and also collect fresh tissue from other prostatic specimens are discussed. The ethical challenges of collecting research tissue without compromising patient care are discussed.
Prostate cancer tissue banks, particularly of frozen tissue are still relatively few in number. Enhanced collection methods which do not prohibit full pathological examination are available but require expertise to maximize their potential.
从前列腺组织中采集用于研究的组织在癌症的鉴定和保存病理材料方面带来了独特的挑战。
福尔马林固定石蜡包埋（FFPE）组织的价值和用途：福尔马林固定石蜡包埋（FFPE）组织在病理处理和标本报告后通常大量存在，但对于详细的分子检测价值有限。如果仔细进行组织微阵列，它是一种有用的技术，可以通过免疫组织化学或荧光原位杂交试验检查许多 FFPE 标本。
在福尔马林固定之前收集新鲜组织，然后对新鲜前列腺癌进行验证样本，这很困难，因为前列腺癌在前列腺标本的切割面上很难宏观识别。此外，在新鲜状态下操作和解剖腺体而不损害手术切缘具有挑战性。讨论了用于解剖新鲜前列腺和从其他前列腺标本中收集新鲜组织的方法。还讨论了在不影响患者护理的情况下收集研究组织的伦理挑战。
前列腺癌组织库，特别是冷冻组织库的数量仍然相对较少。虽然已经有不禁止全面病理检查的增强采集方法，但需要专门知识才能最大限度地发挥其潜力。
