胸腺醌对 Balb/c 小鼠卵清蛋白诱导的过敏性结膜炎的改善作用。

Ameliorative effect of thymoquinone on ovalbumin-induced allergic conjunctivitis in Balb/c mice.

机构信息

Department of Ophthalmology, Second Affiliated Hospital, College of Medicine Xi'an Jiaotong University, Shaanxi, People's Republic of China.

出版信息

Curr Eye Res. 2011 Jul;36(7):591-8. doi: 10.3109/02713683.2011.573898. Epub 2011 May 23.

DOI:10.3109/02713683.2011.573898

PMID:21604966

Abstract

OBJECTIVE

Thymoquinone (TQ) is an active and potent compound in the oil of Nigella sativa, which has anti-inflammatory properties. The aim of this study was to evaluate the effects of TQ on ovalbumin (OVA)-induced allergic conjunctivitis (AC) in Balb/c.

METHODS

The mice were divided into seven experimental groups; PBS Con, OVA Con, Conj, 0.05%TQ, 0.1%TQ, 0.5%TQ and Dex group. The mice were immunized and exposed to OVA, and eyes were treated with TQ and dexamethasone. Ocular symptoms were observed after last exposure to OVA. Eosinophils count in blood and Ophthalmic lavage fluid (OLF), recruitment of inflammatory cells in conjunctiva, serum IgE and OVA-specific IgE were evaluated by Giemsa and HE staining, and Enzyme-Linked Immunosorbent Assay (ELISA) respectively. High Performance Liquid Chromatography (HPLC) was used to determine the histamine level in OLF. The mRNA expression and protein level of cytokines were examined by real time RT-PCR and ELISA respectively.

RESULTS

Ocular symptoms of AC and other characteristics of allergic inflammation including IgE and OVA-specific immunoglobulin E (IgE) level, recruitment of eosinophils, histamine level, mRNA expressions and protein level of cytokines were remarkably increased in OVA-exposed mice compared with the control groups. Administration of TQ suppressed the ocular symptoms, inflammatory cell infiltration in conjunctiva, blood and OLF, increased level of serum IgE and OVA-specific IgE, and OLF histamine level in OVA-exposed mice. Furthermore, TQ abrogated the mRNA expression and serum level of interleukin including 1L-4, IL-5, IL-13 and transforming growth factor beta (TGF-β) in mice immunized and exposed to OVA.

CONCLUSION

Administration of TQ significantly reduced the ocular symptoms in AC by attenuating the recruitment of eosinophils, level of IgE, histamine and cytokines. Our findings suggest that TQ might be a useful intimation for the treatment and future research for AC.

摘要

目的

百里香醌（TQ）是黑种草籽油中的一种活性和有效化合物，具有抗炎特性。本研究旨在评估 TQ 对 Balb/c 卵清蛋白（OVA）诱导的过敏性结膜炎（AC）的影响。

方法

将小鼠分为七组：PBS 对照、OVA 对照、对照、0.05%TQ、0.1%TQ、0.5%TQ 和地塞米松组。将小鼠免疫并暴露于 OVA 中，并用 TQ 和地塞米松治疗眼睛。在最后一次暴露于 OVA 后观察眼部症状。通过吉姆萨和 HE 染色评估血液和眼灌洗液（OLF）中的嗜酸性粒细胞计数、结膜中炎症细胞的募集、血清 IgE 和 OVA 特异性 IgE，酶联免疫吸附试验（ELISA）分别。高效液相色谱法（HPLC）用于测定 OLF 中的组胺水平。通过实时 RT-PCR 和 ELISA 分别检测细胞因子的 mRNA 表达和蛋白水平。

结果

与对照组相比，OVA 暴露的小鼠出现 AC 眼部症状和其他过敏炎症特征，包括 IgE 和 OVA 特异性免疫球蛋白 E（IgE）水平、嗜酸性粒细胞募集、组胺水平、细胞因子的 mRNA 表达和蛋白水平均显著升高。TQ 给药抑制了 OVA 暴露小鼠的眼部症状、结膜、血液和 OLF 中的炎症细胞浸润、血清 IgE 和 OVA 特异性 IgE 水平以及 OLF 组胺水平。此外，TQ 阻断了免疫和暴露于 OVA 的小鼠中白细胞介素包括 1L-4、IL-5、IL-13 和转化生长因子β（TGF-β）的 mRNA 表达和血清水平。