Dentistry School, São Francisco University, Bragança Paulista, SP, Brazil.
Mycoses. 2011 Sep;54(5):e506-12. doi: 10.1111/j.1439-0507.2010.01968.x. Epub 2011 May 23.
This in vitro study evaluated different concentrations of chlorhexidine (CHX) solution on the disinfection of dentures colonised with a reference (ATCC 90028) and azole-resistant (R1, R2 e R3) strains of Candida albicans. Sterile dentures were individually inoculated with one of the strains and incubated at 37 °C for 24 h. Then, each denture was immersed in sterile saline (control) or CHX (2%, 1% or 0.2%) for 10 min. Samples of serial dilutions were spread on Agar Sabouraud Dextrose and incubated at 37 °C for 48 h. The colonies were counted and the values of log(cfu ml(-1)) were analysed by Kruskal-Wallis test (P < 0.05). Dentures immersed in CHX were incubated for 7 days. For all strains, the cfu ml(-1) values of 0.2% CHX were significantly higher than those of 2% and 1% CHX. There was no difference between the cfu ml(-1) values of 2% and 1% CHX. For dentures immersed in CHX, ATCC 90028 strain showed lower cfu ml(-1) values than R2 and R3 strains. For control dentures, cfu ml(-1) values of ATCC 90028 strain were higher than those of R strains. Immersion in 2% CHX resulted in the highest number of dentures without fungal growth after 7 days. For denture disinfection, 2% CHX was the most effective concentration, and R strains were less susceptible to disinfection. Chlorhexidine is effective in disinfection of dentures contaminated with azole-resistant C. albicans.
本体外研究评估了不同浓度的洗必泰（CHX）溶液对受参考（ATCC 90028）和唑类耐药（R1、R2 和 R3）白色念珠菌菌株污染的义齿的消毒效果。无菌义齿分别接种一种菌株，并在 37°C 孵育 24 小时。然后，将每个义齿浸入无菌生理盐水（对照）或 CHX（2%、1%或 0.2%）中 10 分钟。对连续稀释的样本进行琼脂沙保罗葡萄糖琼脂扩散，并在 37°C 孵育 48 小时。对菌落进行计数，并通过 Kruskal-Wallis 检验分析 log(cfu ml(-1))值（P<0.05）。将浸入 CHX 的义齿孵育 7 天。对于所有菌株，0.2%CHX 的 cfu ml(-1)值明显高于 2%和 1%CHX。2%和 1%CHX 的 cfu ml(-1)值之间没有差异。对于浸入 CHX 的义齿，ATCC 90028 菌株的 cfu ml(-1)值低于 R2 和 R3 菌株。对于对照义齿，ATCC 90028 菌株的 cfu ml(-1)值高于 R 菌株。7 天后，浸入 2%CHX 的义齿中没有真菌生长的数量最多。对于义齿消毒，2%CHX 是最有效的浓度，R 菌株对消毒的敏感性较低。洗必泰对受唑类耐药白色念珠菌污染的义齿具有消毒作用。
