Ganesh S, Gujjari Anil Kumar, S Shylesh Kumar B, B Ravi M, S Sowmya, S Meenakshi
Department of Prosthodontics including Crown & Bridge, JSS Dental College and Hospital, A Constituent College of JSS University Mysore, Karnataka, India.
J Int Oral Health. 2013 Jun;5(3):55-62. Epub 2013 Jun 23.
To evaluate and compare the effectiveness of various disinfectants on Candida albicans (C.albicans) and Staphylococcus aureus (Staph.aureus) inoculated on acrylic denture base resin and effect of disinfectants on flexural strength of denture base resin.
MATERIALS & METHODS: A total of 130 acrylic denture base resin specimens were fabricated and processed according to manufacturer instructions. 82 sterile specimens were used for microbiological study. 2 specimens were cultured for organism growth to ensure sterility. 40 sterile specimens each were inoculated by immersing in Sabouraud & Nutrient broth containing microorganisms for 45 minutes each. Then the specimens were immersed in chlorhexidine, glutaraldehyde & distilled water (control) for 4 & 8 minutes. Then the specimens were neutralized. After neutralization the specimens were cultured onto Sabouraud's broth for C.albicans and Nutrient broth for Staph.aureus incubated for 72 h and observed for turbidity. At the end of 72 h subculture were made onto Sabourads dextrose agar media for C.albicans, Blood agar media for Staph.aureus and incubated for 48 h to observe growth. For flexural strength testing, 8 specimens each was immersed in the above mentioned disinfectants and distilled water for 8 & 16 minutes. Each of which was then subjected to 3 point flexural load in Lloyd's Universal testing machine. The peak load was recorded and flexural strength values were calculated.
The microbiological study revealed that both disinfectants were equally effective at 4 minutes against C.albicans & Staph.aureus microorganisms. Flexural strength test revealed no significant difference between test and control groups.
Chlorhexdine and Glutaraldehyde disinfectans are equally effective against C.albicans and Staph.aureus microorganisms. Heat polymerized acrylic denture base resin did not demonstrate any significant change in flexural strength between control and test specimens. How to cite this article: Ganesh S, Gujjari A K, Shylesh K B S, Ravi M B, Sowmya S, Meenakshi S.Comparative Study to Assess the Effectiveness of Various Disinfectants on two Microorganisms and the effect of same on Flexural Strength of Acrylic Denture Base Resin - An In Vitro Study. J Int Oral Health 2013; 5(3):55-62.
评估并比较各种消毒剂对接种于丙烯酸义齿基托树脂上的白色念珠菌和金黄色葡萄球菌的有效性,以及消毒剂对义齿基托树脂弯曲强度的影响。
共制作130个丙烯酸义齿基托树脂标本,并按照制造商说明进行处理。82个无菌标本用于微生物学研究。培养2个标本以确保无菌生长。将40个无菌标本分别浸入含有微生物的沙氏肉汤和营养肉汤中45分钟进行接种。然后将标本分别浸入洗必泰、戊二醛和蒸馏水(对照)中4分钟和8分钟。之后对标本进行中和处理。中和后,将标本接种于用于白色念珠菌的沙氏肉汤和用于金黄色葡萄球菌的营养肉汤中,培养72小时并观察浑浊情况。72小时结束时,转种至用于白色念珠菌的沙氏葡萄糖琼脂培养基和用于金黄色葡萄球菌的血琼脂培养基中,培养48小时以观察生长情况。对于弯曲强度测试,将8个标本分别浸入上述消毒剂和蒸馏水中8分钟和16分钟。然后将每个标本在劳埃德万能试验机上进行三点弯曲加载。记录峰值载荷并计算弯曲强度值。
微生物学研究表明,两种消毒剂在4分钟时对白色念珠菌和金黄色葡萄球菌微生物的效果相同。弯曲强度测试表明测试组和对照组之间无显著差异。
洗必泰和戊二醛消毒剂对白色念珠菌和金黄色葡萄球菌微生物的效果相同。热聚合丙烯酸义齿基托树脂在对照标本和测试标本之间的弯曲强度未显示任何显著变化。如何引用本文:Ganesh S, Gujjari A K, Shylesh K B S, Ravi M B, Sowmya S, Meenakshi S.比较研究评估各种消毒剂对两种微生物的有效性及其对丙烯酸义齿基托树脂弯曲强度的影响——一项体外研究。《国际口腔健康杂志》2013年;5(3):55 - 62。