Chair and Department of Pharmacology, Medical University of Silesia, ul. Jordana 19, 41-808 Zabrze, Poland.
Pharmacol Res. 2011 Sep;64(3):218-25. doi: 10.1016/j.phrs.2011.04.011. Epub 2011 May 5.
Branched chain amino acids (BCAA) have been shown to have a general protective effect on the heart in different animal models as well as in humans. However, so far no attempt has been made to specifically elucidate their influence on arrhythmias. Our study was performed to evaluate whether an infusion of either l-leucine or l-valine in a dose of 1mgkg(-1)h(-1) 10min before a 7-min period of left anterior descending artery occlusion followed by 15min of reperfusion, had an effect on arrhythmias measured during the reperfusion phase in the ischemia- and reperfusion-induced arrhythmias model in rats in vivo. The effect of the infusion of these substances on mean arterial blood pressure was monitored throughout the experiment. Both of the tested amino acids exhibited significant antiarrhythmic properties. l-Leucine reduced the duration of ventricular fibrillation (P<0.05) and l-valine decreased the duration of ventricular fibrillation (P<0.001) and ventricular tachycardia (P<0.05). The two amino acids were generally hypotensive. l-Valine lowered blood pressure in all phases of the experiment (P<0.05) while l-leucine lowered this parameter mainly towards the end of occlusion and reperfusion (P<0.05). In addition, 30min infusion of the amino acids in the used dose did not produce any apparent adverse histological changes that were remarkably different from control. In summary, the results of our study suggest that l-leucine and l-valine in the dose that was used attenuates arrhythmias and are hypotensive in their influence. Our findings lend support to the many ongoing investigations into the benefit of the application of l-leucine and l-valine in cardiology like their addition to cardioplegic solutions.
支链氨基酸（BCAA）已被证明在不同的动物模型以及人类中对心脏具有普遍的保护作用。然而，迄今为止，尚未有人试图专门阐明它们对心律失常的影响。我们的研究旨在评估在左前降支闭塞 7 分钟后，再灌注前 10 分钟以 1mgkg(-1)h(-1)的剂量输注 l-亮氨酸或 l-缬氨酸，是否会对体内缺血再灌注诱导的心律失常大鼠模型再灌注期间测量的心律失常产生影响。在整个实验过程中监测输注这些物质对平均动脉血压的影响。两种测试的氨基酸都表现出明显的抗心律失常特性。l-亮氨酸减少心室颤动的持续时间（P<0.05），l-缬氨酸减少心室颤动（P<0.001）和室性心动过速（P<0.05）的持续时间。两种氨基酸通常具有降压作用。l-缬氨酸在实验的所有阶段都降低血压（P<0.05），而 l-亮氨酸主要在闭塞和再灌注结束时降低该参数（P<0.05）。此外，在使用的剂量下，30 分钟输注氨基酸不会产生任何明显的组织学变化，这些变化与对照明显不同。总之，我们的研究结果表明，在使用的剂量下，l-亮氨酸和 l-缬氨酸可减轻心律失常并降低血压。我们的发现支持许多正在进行的关于应用 l-亮氨酸和 l-缬氨酸的益处的研究，如将其添加到心脏停搏液中。
