College of Chemistry, Jilin University, Changchun 130012, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Jun 15;879(20):1833-8. doi: 10.1016/j.jchromb.2011.05.009. Epub 2011 May 11.
Shikonin and β,β'-dimethylacrylshikonin in Arnebia euchroma (Royle) Johnst. were extracted by ionic liquid-based ultrasonic-assisted extraction (IL-based UAE) and determined by high-performance liquid chromatography (HPLC). The dried powder of A. euchroma (Royle) Johnst. was mixed with a room temperature ionic liquid [C(6)MIM][BF(4)] to form a suspension, and then the ultrasonic extraction was performed in a water bath at ambient temperature. The calibration curve showed good linear relationship (r>0.9998) in the concentration range of 1.75-140 μg/mL for shikonin and 2.15-1360 μg/mL for β,β'-dimethylacrylshikonin. The recoveries were between 69.79% and 82.35%. The IL-based UAE is free of volatile organic solvents, and consumes less sample, time and solvent, compared with regular ultrasonic and Soxhlet extraction. There was no obvious difference in the extraction yields of active constitutions obtained by the three extraction methods.
紫草素和β，β'-二甲基丙烯酰紫草素在Arnebia euchroma（Royle）Johnst.中用离子液体-超声辅助提取（IL-UAE）提取，并通过高效液相色谱（HPLC）测定。将干燥的 A. euchroma（Royle）Johnst.粉末与室温离子液体[C（6）MIM][BF（4）]混合形成悬浮液，然后在环境温度的水浴中进行超声提取。校准曲线显示紫草素在 1.75-140 μg/mL 浓度范围内和β，β'-二甲基丙烯酰紫草素在 2.15-1360 μg/mL 浓度范围内具有良好的线性关系（r>0.9998）。回收率在 69.79%至 82.35%之间。与常规超声和索氏提取相比，基于离子液体的 UAE 无需使用挥发性有机溶剂，且消耗的样品、时间和溶剂更少。三种提取方法获得的活性成分的提取率没有明显差异。
