Tan Zhi-Jian, Wang Chao-Yun, Yang Zi-Zhen, Yi Yong-Jian, Wang Hong-Ying, Zhou Wan-Lai, Li Fen-Fang
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Molecules. 2015 Sep 30;20(10):17929-43. doi: 10.3390/molecules201017929.
In this work, a two-step extraction methodology of ionic liquid-based ultrasonic-assisted extraction (IL-UAE) and ionic liquid-based aqueous two-phase system (IL-ATPS) was developed for the extraction and purification of secoisolariciresinol diglucoside (SDG) from flaxseed. In the IL-UAE step, several kinds of ILs were investigated as the extractants, to identify the IL that affords the optimum extraction yield. The extraction conditions such as IL concentration, ultrasonic irradiation time, and liquid-solid ratio were optimized using response surface methodology (RSM). In the IL-ATPS step, ATPS formed by adding kosmotropic salts to the IL extract was used for further separation and purification of SDG. The most influential parameters (type and concentration of salt, temperature, and pH) were investigated to obtain the optimum extraction efficiency. The maximum extraction efficiency was 93.35% under the optimal conditions of 45.86% (w/w) IL and 8.27% (w/w) Na₂SO₄ at 22 °C and pH 11.0. Thus, the combination of IL-UAE and IL-ATPS makes up a simple and effective methodology for the extraction and purification of SDG. This process is also expected to be highly useful for the extraction and purification of bioactive compounds from other important medicinal plants.
在本研究中，开发了一种两步萃取方法，即基于离子液体的超声辅助萃取（IL-UAE）和基于离子液体的双水相体系（IL-ATPS），用于从亚麻籽中提取和纯化开环异落叶松脂素二葡萄糖苷（SDG）。在IL-UAE步骤中，研究了几种离子液体作为萃取剂，以确定能提供最佳萃取产率的离子液体。使用响应面法（RSM）优化了离子液体浓度、超声辐照时间和液固比等萃取条件。在IL-ATPS步骤中，通过向离子液体提取物中添加促盐析盐形成的双水相体系用于SDG的进一步分离和纯化。研究了最具影响力的参数（盐的类型和浓度、温度和pH值）以获得最佳萃取效率。在45.86%（w/w）离子液体和8.27%（w/w）Na₂SO₄、22℃和pH 11.0的最佳条件下，最大萃取效率为93.35%。因此，IL-UAE和IL-ATPS的组合构成了一种简单有效的SDG提取和纯化方法。该工艺预计对从其他重要药用植物中提取和纯化生物活性化合物也非常有用。
