Kaczynska Katarzyna, Walski Michał, Szereda-Przestaszewska Małgorzata
Laboratory of Respiratory Reflexes, PAS Mossakowski Medical Research Centre, Pawinskiego Str. 5, 02-106 Warsaw, Poland.
J Electron Microsc (Tokyo). 2011;60(4):289-94. doi: 10.1093/jmicro/dfr035. Epub 2011 May 22.
Pulmonary toxicity of lead was studied in rats after an intraperitoneal administration of lead acetate at a dose of 25 mg/kg. Three consecutive days of treatment increased lead content in the whole blood to 2.1 µg/dl and in lung homogenate it attained 9.62 µg/g w.w. versus control values of 0.17 µg/dl and 0.78 µg/g w.w., respectively. At the ultrastructural level, the effects of lead toxicity were observed in lung capillaries, interstitium, epithelial cells and alveolar lining layer. Accumulation of aggregated platelets, leucocytic elements and monocytes was found within capillaries. Interstitium comprised a substantial number of collagen, elastin filaments and lipofibroblasts. Lamellar bodies of type II pneumocytes contained phospolipid lamellae, which stratified into an irregular arrangement. Pulmonary alveoli were filled with macrophages. The extracellular lining layer of lung alveoli was partially destroyed. This study provided evidence that acute lead intoxication affects the whole lung parenchyma and by impairing production of the surfactant might disturb the regular respiratory function.
在大鼠腹腔注射剂量为25mg/kg的醋酸铅后，对铅的肺毒性进行了研究。连续三天的治疗使全血中的铅含量增加到2.1μg/dl，肺匀浆中的铅含量达到9.62μg/g湿重，而对照组的值分别为0.17μg/dl和0.78μg/g湿重。在超微结构水平上，在肺毛细血管、间质、上皮细胞和肺泡衬里层观察到了铅毒性的影响。在毛细血管内发现了聚集的血小板、白细胞成分和单核细胞。间质包含大量的胶原蛋白、弹性纤维和脂成纤维细胞。II型肺细胞的板层小体含有磷脂板层，这些板层分层形成不规则排列。肺泡充满了巨噬细胞。肺泡的细胞外衬里层部分被破坏。这项研究提供了证据，表明急性铅中毒会影响整个肺实质，并通过损害表面活性剂的产生可能会干扰正常的呼吸功能。
