人类恶性淋巴瘤和霍奇金病中免疫球蛋白、T细胞受体及bcr重排的分析

Analysis of immunoglobulin, T cell receptor and bcr rearrangements in human malignant lymphoma and Hodgkin's disease.

作者信息

Tesch H, May P, Krueger G R, Fischer R, Diehl V

机构信息

First Medical Clinic, University of Cologne, FRG.

出版信息

Oncology. 1990;47(3):215-23. doi: 10.1159/000226819.

DOI:10.1159/000226819

PMID:2160631

Abstract

Rearrangements of immunoglobulin and T cell receptor (TCR) genes have been demonstrated in malignant lymphoid tumors of B and T cell origin. In Philadelphia chromosome-positive chronic myeloid leukemia and acute lymphocytic leukemia cells the bcr and c-abl genes are reorganized and transcripts composed of both genes are expressed. We analyzed the organization of bcr, immunoglobulin and TCR genes in malignant lymphomas. Our data show that in all B cell lymphomas analyzed the JH genes and in some cases also the J kappa genes were rearranged. In a Burkitt lymphoma and in a Kil lymphoma distinct rearranged TCR gamma fragments were detected, in a second Burkitt lymphoma two rearranged TCR beta gene fragments occurred together with a rearranged JH gene fragment. In two T cell lymphomas rearranged TCR beta genes were observed; one of these lymphomas also carried rearranged TCR gamma and JH genes. In Hodgkin's disease in 3 out of 7 cases rearranged immunoglobulin genes were detected. In 1 case, which was diagnosed as a follicular hyperplasia, rearranged JH and TCR gamma fragments appeared. In none of the analyzed lymphomas could bcr rearrangements be observed.

摘要

免疫球蛋白和T细胞受体（TCR）基因重排已在B细胞和T细胞来源的恶性淋巴瘤中得到证实。在费城染色体阳性的慢性粒细胞白血病和急性淋巴细胞白血病细胞中，bcr和c-abl基因发生重排，由这两个基因组成的转录本得以表达。我们分析了恶性淋巴瘤中bcr、免疫球蛋白和TCR基因的组织情况。我们的数据显示，在所有分析的B细胞淋巴瘤中，JH基因发生了重排，在某些情况下，Jκ基因也发生了重排。在一例伯基特淋巴瘤和一例基尔淋巴瘤中检测到了不同的重排TCRγ片段，在另一例伯基特淋巴瘤中，两个重排的TCRβ基因片段与一个重排的JH基因片段同时出现。在两例T细胞淋巴瘤中观察到了重排的TCRβ基因；其中一例淋巴瘤还携带重排的TCRγ和JH基因。在霍奇金病中，7例中有3例检测到了重排的免疫球蛋白基因。在1例被诊断为滤泡性增生的病例中，出现了重排的JH和TCRγ片段。在所分析的淋巴瘤中均未观察到bcr重排。