霍奇金淋巴瘤中抗原受体基因的分析。
Analysis of antigen receptor genes in Hodgkin's disease.
作者信息
Angel C A, Pringle J H, Naylor J, West K P, Lauder I
机构信息
Department of Pathology, University of Sheffield Medical School.
出版信息
J Clin Pathol. 1993 Apr;46(4):337-40. doi: 10.1136/jcp.46.4.337.
AIM
To analyse the configuration of the antigen receptor genes in Hodgkin's disease.
METHODS
DNA extracted from 45 samples of Hodgkin's disease was analysed using Southern blotting and DNA hybridisation, using probes to the joining region of the immunoglobulin heavy chain gene, the constant region of kappa immunoglobulin light chain gene, and the constant region of the beta chain of the T cell receptor gene.
RESULTS
A single case of nodular sclerosing disease showed clonal rearrangement of the immunoglobulin heavy and light chain genes, all other samples having germline immunoglobulin genes. The nature of the clonal population in the diseased tissue is uncertain, because the intensity of the rearranged bands did not correlate with the percentage of Reed-Sternberg cells present. The T cell receptor genes were in germline configuration in all the samples.
CONCLUSIONS
Antigen receptor gene rearrangement is a rare finding in unselected cases of Hodgkin's disease.
目的
分析霍奇金淋巴瘤中抗原受体基因的构型。
方法
采用Southern印迹法和DNA杂交技术，使用针对免疫球蛋白重链基因连接区、κ免疫球蛋白轻链基因恒定区以及T细胞受体基因β链恒定区的探针，对45例霍奇金淋巴瘤样本提取的DNA进行分析。
结果
1例结节硬化型病例显示免疫球蛋白重链和轻链基因发生克隆性重排，其他所有样本的免疫球蛋白基因均为种系构型。病变组织中克隆群体的性质尚不确定，因为重排条带的强度与里德-斯腾伯格细胞的百分比无关。所有样本的T细胞受体基因均为种系构型。
结论
在未经选择的霍奇金淋巴瘤病例中，抗原受体基因重排是一种罕见的发现。
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