Sambuu Rentsenkhand, Takagi Mitsuhiro, Namula Zhao, Otoi Takeshige, Shiga Satoshi, Rodrigues Dos Santos Regiane, Fink-Gremmels Johanna
United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
J Reprod Dev. 2011 Sep;57(4):547-50. doi: 10.1262/jrd.11-026h. Epub 2011 May 23.
The influence of acute exposure to zearalenone (ZEN) on porcine oocyte maturation, fertilization or sperm penetration ability during both in vitro maturation and fertilization was evaluated. First, oocytes were cultured in ZEN-containing (0-1000 µg/l) maturation medium and then fertilized. The oocytes maturing in vitro without ZEN were then fertilized in ZEN-containing fertilization medium. The maturation rates of oocytes and penetration ability of sperm decreased significantly in the presence of 1000 µg/l of ZEN. However, neither increases in the rates of degeneration and DNA fragmentation of oocytes nor reductions in normal and polyspermic fertilization were observed. ZEN did not affect the sperm penetration rates; however, 1000 µg/l ZEN had positive effects on normal and polyspermic fertilization rates. Therefore, it can be suggested that an acute exposure of porcine oocytes during maturation and of oocytes and sperm during fertilization to ZEN up to 1000 µg/l may not affect the fertility of the oocytes.
评估了急性暴露于玉米赤霉烯酮（ZEN）对体外成熟和受精过程中猪卵母细胞成熟、受精或精子穿透能力的影响。首先，将卵母细胞培养在含ZEN（0 - 1000 µg/l）的成熟培养基中，然后进行受精。接着，将未接触ZEN体外成熟的卵母细胞在含ZEN的受精培养基中受精。当存在1000 µg/l ZEN时，卵母细胞的成熟率和精子的穿透能力显著降低。然而，未观察到卵母细胞退化率和DNA片段化增加，也未观察到正常受精和多精受精率降低。ZEN不影响精子穿透率；然而，1000 µg/l ZEN对正常受精和多精受精率有积极影响。因此，可以认为，猪卵母细胞在成熟过程中以及卵母细胞和精子在受精过程中急性暴露于高达1000 µg/l的ZEN可能不会影响卵母细胞的生育能力。
