Takagi Mitsuhiro, Mukai Shuhei, Kuriyagawa Toshiyuki, Takagaki Katsuhito, Uno Seiichi, Kokushi Emiko, Otoi Takeshige, Budiyanto Agung, Shirasuna Koumei, Miyamoto Akio, Kawamura Osamu, Okamoto Koji, Deguchi Eisaburo
Faculty of Agriculture, Kagoshima University, Kagoshima, Japan.
Reprod Toxicol. 2008 Oct;26(2):164-9. doi: 10.1016/j.reprotox.2008.08.006. Epub 2008 Aug 28.
Zearalenone (Zen) and its metabolites are estrogenic and may be important factors involved in reproductive disorders in domestic animals. We aimed to (1) simultaneously detect Zen and its metabolites in bovine follicular fluids (FFs) by liquid chromatography-tandem mass spectrometry and (2) examine the in vitro effects of Zen on bovine oocytes. Zen and its metabolites were detected in 6 of 32 normal follicles and 7 of 20 cystic follicles. Bovine oocytes were cultured in a maturation media containing various Zen concentrations (0 [control], 1, 10, 100, and 1000microg/L), fertilized, and cultured further. Maturation rates decreased dose-dependently. Further, maturation of 62 (50%) of 124 oocytes examined in the 1000-microg/L group was arrested in metaphase I, without affecting the fertilization rate. Blastocyst-formation rates did not significantly differ among the groups. Zen and its metabolites were detectable in bovine FFs. High Zen concentration may adversely affect meiotic competence but not the fertilization and development rates.
玉米赤霉烯酮（ZEN）及其代谢产物具有雌激素活性，可能是导致家畜生殖紊乱的重要因素。我们旨在：（1）通过液相色谱 - 串联质谱法同时检测牛卵泡液（FFs）中的ZEN及其代谢产物；（2）研究ZEN对牛卵母细胞的体外影响。在32个正常卵泡中的6个以及20个囊性卵泡中的7个中检测到了ZEN及其代谢产物。将牛卵母细胞培养在含有不同ZEN浓度（0[对照]、1、10、100和1000μg/L）的成熟培养基中，进行受精并进一步培养。成熟率呈剂量依赖性下降。此外，在1000μg/L组检测的124个卵母细胞中有62个（50%）在减数第一次分裂中期停滞，且不影响受精率。各组间囊胚形成率无显著差异。在牛卵泡液中可检测到ZEN及其代谢产物。高浓度的ZEN可能会对减数分裂能力产生不利影响，但不会影响受精率和发育率。
