Patel Pk, Patel Kv, Gandhi Tr
Department of Pharmacology, C. K. Pithawalla Institute of Pharmaceutical Science and Research, Surat - 395 007, Gujarat, India.
J Young Pharm. 2011 Jan;3(1):41-7. doi: 10.4103/0975-1483.76418.
The present investigation was undertaken to evaluate the bronchodilating effect and bronchial hyperreactivity of alcoholic extract of Taxus baccata Linn. (AET) leaves in experimental animals. Bronchodilator activity of AET was studied on the histamine and acetylcholine aerosol induced bronchospasm in guinea pigs and bronchial hyperreactivity was studied on bronchoalveolar lavage fluid (BALF) in the egg albumin sensitized guinea pigs and by histopathological studies. In vitro mast cell stabilizing activity was studied using compound 48/80 as a degranulating agent. Treatment with AET (200 and 400 mg/kg, p.o., for 7 days) showed significant protection against histamine and acetylcholine aerosol induced bronchospasm in guinea pigs. Significant decrease in the total leukocyte and differential leukocyte count in the BALF of the egg albumin sensitized guinea pigs was observed by administration of AET (200 and 400 mg/kg, p.o., for 15 days). AET dose dependently protected the mast cell disruption induced by compound 48/80. These results suggest that AET not only has bronchodilating activity but also decreases bronchial hyperreactivity by decreasing the infiltration of inflammatory cells in the airway and inhibiting the release of histamine like mediators from the mast cell by stabilizing it.
本研究旨在评估欧洲红豆杉叶乙醇提取物（AET）在实验动物中的支气管扩张作用和支气管高反应性。在豚鼠中，研究了AET对组胺和乙酰胆碱气雾剂诱导的支气管痉挛的支气管扩张活性；在卵清蛋白致敏的豚鼠中，通过支气管肺泡灌洗液（BALF）和组织病理学研究，研究了支气管高反应性。使用化合物48/80作为脱颗粒剂，研究了体外肥大细胞稳定活性。用AET（200和400mg/kg，口服，持续7天）治疗对豚鼠组胺和乙酰胆碱气雾剂诱导的支气管痉挛具有显著的保护作用。通过给予AET（200和400mg/kg，口服，持续15天），观察到卵清蛋白致敏豚鼠BALF中总白细胞和分类白细胞计数显著降低。AET剂量依赖性地保护化合物48/80诱导的肥大细胞破坏。这些结果表明，AET不仅具有支气管扩张活性，还通过减少气道中炎症细胞的浸润和稳定肥大细胞来抑制组胺样介质的释放，从而降低支气管高反应性。
