Jean-Marie Ramirez, Qiang Bai, Marilyne Dijon-Grinand, Said Assou, Samir Hamamah, John De Vos, INSERM, U847, Montpellier, F 34000, France.
World J Stem Cells. 2010 Apr 26;2(2):24-33. doi: 10.4252/wjsc.v2.i2.24.
Human pluripotent stem cells (PSCs), encompassing embryonic stem cells and induced pluripotent stem cells, proliferate extensively and differentiate into virtually any desired cell type. PSCs endow regenerative medicine with an unlimited source of replacement cells suitable for human therapy. Several hurdles must be carefully addressed in PSC research before these theoretical possibilities are translated into clinical applications. These obstacles are: (1) cell proliferation; (2) cell differentiation; (3) genetic integrity; (4) allogenicity; and (5) ethical issues. We discuss these issues and underline the fact that the answers to these questions lie in a better understanding of the biology of PSCs. To contribute to this aim, we have developed a free online expression atlas, Amazonia!, that displays for each human gene a virtual northern blot for PSC samples and adult tissues (http://www.amazonia.transcriptome.eu).
人类多能干细胞（PSCs）包括胚胎干细胞和诱导多能干细胞，它们广泛增殖并分化为几乎任何所需的细胞类型。PSCs 为再生医学提供了无限的替代细胞来源，适用于人类治疗。在这些理论可能性转化为临床应用之前，PSC 研究必须仔细解决几个障碍。这些障碍是：（1）细胞增殖；（2）细胞分化；（3）遗传完整性；（4）同种异体；和（5）伦理问题。我们讨论了这些问题，并强调了这样一个事实，即这些问题的答案在于更好地了解 PSCs 的生物学特性。为了实现这一目标，我们开发了一个免费的在线表达图谱 Amazonia！，它为 PSC 样本和成人组织中的每个人类基因显示了一个虚拟的 northern blot（http://www.amazonia.transcriptome.eu）。
