Yonemura Y, Ninomiya I, Nojima N, Sugiyama K, Fujimura T, Tsuchihara K, Kawamura T, Kaji M, Miyazaki I, Endo Y, Tanaka M, Sasaki T
KANAZAWA UNIV,CANC RES INST,DEPT EXPTL THERAPEUT,KANAZAWA,ISHIKAWA 920,JAPAN.
Oncol Rep. 1994 Jul;1(4):765-72. doi: 10.3892/or.1.4.765.
To elucidate the relation between urokinase-type plasminogen activator (U-PA) and metastasis in gastric cancer, we examined U-PA tissue status immunohistochemically. Ninety-eight primary gastric cancer, prepared by AMeX method, were analyzed with anti-U-PA and anti-proliferating cell nuclear antigen (PCNA) monoclonal antibody. In addition, DNA ploidy patterns were determined by cytofluorometer after staining with propidium iodide. U-PA immunoreactivity can be observed as diffuse cytoplasmic staining, as intensely outlined luminal borders or in desquameted cells in the lumens. U-PA expression-positive tumors showed higher incidence of serosal invasion, lymph node involvement or larger tumors than did U-PA negative ones. There was no correlation between U-PA tissue status and PCNA labeling rates or DNA ploidy patterns. Patients with a U-PA positive tumor survived significantly shorter than those with U-PA negative ones. These results may indicate that U-PA tissue status is a useful biological prognostic indicator in gastric cancer. The high malignant potential of U-PA positive tumors may be associated with a rapid infiltrating capacity through gastric wall but not with a high proliferative activity.
为阐明尿激酶型纤溶酶原激活物（U-PA）与胃癌转移之间的关系，我们采用免疫组织化学方法检测了U-PA的组织状态。采用AmeX法制备了98例原发性胃癌标本，并用抗U-PA和抗增殖细胞核抗原（PCNA）单克隆抗体进行分析。此外，用碘化丙啶染色后通过细胞荧光计测定DNA倍体模式。U-PA免疫反应性可表现为弥漫性细胞质染色、强烈勾勒出的管腔边界或管腔内脱落细胞中的染色。与U-PA阴性肿瘤相比，U-PA表达阳性的肿瘤浆膜侵犯、淋巴结受累的发生率更高或肿瘤更大。U-PA组织状态与PCNA标记率或DNA倍体模式之间无相关性。U-PA阳性肿瘤患者的生存期明显短于U-PA阴性患者。这些结果可能表明，U-PA组织状态是胃癌有用的生物学预后指标。U-PA阳性肿瘤的高恶性潜能可能与其通过胃壁的快速浸润能力有关，而与高增殖活性无关。
