Yang Xue-Wen, Gao Feng, Chen Ya-Jun, Teng Feng-Meng
Department of Laboratory Medicine, Jiangsu Province Hospital of TCM, Nanjing, 210029, Jiangsu, People's Republic of China.
Cell Biochem Biophys. 2015 Jul;72(3):649-52. doi: 10.1007/s12013-014-0356-z.
The aim of this study is to explore the expression and significance of urokinase-type plasminogen activator (u-PA) and vascular endothelial growth factor (VEGF) in gastric cancer, providing a novel insight for the diagnosis and treatment of gastric cancer. The gastric cancer specimens, which were excised from 87 patients and confirmed during July, 2012-July, 2014, were selected as observation group, and the normal tissue next to the tumor (more than 5 cm from the edge of the tumor) from 45 patients were randomly selected as control. u-PA and VEGF were detected by immunohistochemistry for the analysis of the correlation of u-PA and VEGF in two groups. The positive rates of u-PA and VEGF in gastric cancer tissue were 81.61 and 79.31 %, respectively, which were 6.67 and 8.89 % in the control group, respectively. The positive rates in the observation group were obviously higher than those in the control group, and the difference was statistically significant (P < 0.05). Among the 87 gastric cancer tissue samples from the observation group, the positive rates of u-PA and VEGF in the gastric cancer with poor differentiation, lymphatic metastasis, invasion up to serosal layer, and TNM stage III + IV were obviously higher than those in the gastric cancer with high differentiation, non-lymphatic metastasis, invasion not up to the serosal layer, and TNM stage I + II, and the difference was statistically significant (P < 0.05). Among the 87 gastric cancer tissue samples from the observation group, u-PA and VEGF were found to be positive in 60 cases and negative in 7 cases. By comparing the two groups, u-PA and VEGF were positively correlated in gastric cancer tissue (P < 0.05). u-PA and VEGF were highly expressed in gastric cancer tissue, which could be used as the molecular biological indicators to predict the invasion and metastasis potential of gastric cancer. The combination of two factors plays a guiding role in early diagnosis and treatment of gastric cancer.
本研究旨在探讨尿激酶型纤溶酶原激活剂（u-PA）和血管内皮生长因子（VEGF）在胃癌中的表达及意义，为胃癌的诊断和治疗提供新的思路。选取2012年7月至2014年7月期间手术切除并经病理确诊的87例胃癌患者的癌组织作为观察组，随机选取45例患者肿瘤旁正常组织（距肿瘤边缘5 cm以上）作为对照组。采用免疫组织化学法检测u-PA和VEGF，分析两组u-PA和VEGF的相关性。结果显示，胃癌组织中u-PA和VEGF阳性率分别为81.61%和79.31%，对照组分别为6.67%和8.89%，观察组阳性率明显高于对照组，差异有统计学意义（P<0.05）。观察组87例胃癌组织中，低分化、有淋巴结转移、侵及浆膜层及TNM分期III+IV期胃癌的u-PA和VEGF阳性率明显高于高分化、无淋巴结转移、未侵及浆膜层及TNM分期I+II期胃癌，差异有统计学意义（P<0.05）。观察组87例胃癌组织中，u-PA和VEGF双阳性60例，双阴性7例。两组比较，胃癌组织中u-PA和VEGF呈正相关（P<0.05）。u-PA和VEGF在胃癌组织中高表达，可作为预测胃癌侵袭转移潜能的分子生物学指标，两者联合对胃癌的早期诊断和治疗具有指导意义。
