Key Lab of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072, China.
Biochem Genet. 2011 Oct;49(9-10):656-64. doi: 10.1007/s10528-011-9440-x. Epub 2011 May 24.
A novel ascorbate peroxidase (APX) cDNA was obtained from Nelumbo nucifera (Elian). The phylogenetic analysis indicated that N. nucifera APX grouped together with chloroplastic APX of high plants. The recombinant protein expressed by PET-30a vector showed APX activity (0.04 mM ascorbate min(-1) mg(-1) protein). The APX mRNA was expressed in young leaves, roots, terminal buds, and leafstalks. Synergistic expression of N. nucifera APX and MnSOD mRNA was indicated in the short-term response to mechanical wounding.
从莲（Nelumbo nucifera）中获得了一种新型抗坏血酸过氧化物酶（APX）cDNA。系统发生分析表明，莲 APX 与高等植物的叶绿体 APX 聚为一组。由 PET-30a 载体表达的重组蛋白显示出 APX 活性（0.04 mM 抗坏血酸 min(-1) mg(-1) 蛋白）。APX mRNA 在幼叶、根、顶芽和叶柄中表达。在短期机械损伤响应中，莲 APX 和 MnSOD mRNA 的协同表达得到了表明。
