Key Lab of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan, 430072, China.
Appl Biochem Biotechnol. 2011 Mar;163(5):679-91. doi: 10.1007/s12010-010-9074-1. Epub 2010 Aug 27.
Two cytosolic copper-zinc superoxide dismutase (cytCuZnSOD) complementary deoxyribonucleic acid were achieved in Nelumbo nucifera (Elian). The active sites and common characteristics of cytCuZnSOD family were showed by homology modeling. The two recombinant proteins expressed by PET-32a vector showed the similar SOD activity (89.94 ± 0.54 U/mg) and could maintain more than 90% activity after incubation at 65°C. The subcellular location by green fluorescent protein revealed that these two isoforms were all located in cytosol and nucleus. The cytCuZnSODs were expressed in various parts of N. nucifera, which were expressed highest in the leafstalks and young leaves and lowest in the roots. The cytCuZnSOD messenger ribonucleic acids isolated from wounded leaves significantly increased at 1.5 h after treatment (HAT) with the highest expression at 3 HAT, after which the level decreased.
在莲（Nelumbo nucifera）中获得了两种胞质铜锌超氧化物歧化酶（cytCuZnSOD）的互补脱氧核糖核酸。通过同源建模显示了 cytCuZnSOD 家族的活性位点和共同特征。由 PET-32a 载体表达的两种重组蛋白表现出相似的 SOD 活性（89.94±0.54 U/mg），并且在 65°C 孵育后可以保持超过 90%的活性。通过绿色荧光蛋白进行的亚细胞定位表明，这两种同工酶均位于细胞质和细胞核中。cytCuZnSOD 在莲的各个部位表达，在叶柄和嫩叶中表达最高，在根中表达最低。从受伤叶片中分离出的 cytCuZnSOD 信使核糖核酸在处理后 1.5 小时（HAT）显著增加，在 3 HAT 时表达最高，之后水平下降。
