Huang Yan, Willars Gary B
Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, UK.
Methods Mol Biol. 2011;746:53-84. doi: 10.1007/978-1-61779-126-0_4.
The addition of one or more epitope tags to G-protein-coupled receptors (GPCRs) has facilitated a wide variety of studies on their structure and function. Epitope-tagging is achieved using relatively straightforward molecular techniques but requires careful consideration about the nature of the epitope tag and its location within the receptor. Here, we describe both the strategies and methodologies for the generation of epitope-tagged GPCRs. We highlight a range of possible techniques that depend upon the available starting material, the nature of the epitope to be incorporated, and suggest a strategy to ease the tagging of multiple receptor types.
在G蛋白偶联受体（GPCRs）上添加一个或多个表位标签有助于对其结构和功能进行广泛的研究。使用相对简单的分子技术即可实现表位标签的添加，但需要仔细考虑表位标签的性质及其在受体中的位置。在此，我们描述了生成表位标签化GPCRs的策略和方法。我们重点介绍了一系列可能的技术，这些技术取决于可用的起始材料、要掺入的表位的性质，并提出了一种简化多种受体类型标签添加的策略。
