Thorey Fritz, Weinert Kerstin, Weizbauer Andreas, Witte Frank, Willbold Elmar, Bartsch Ivonne, Hoffmann Andrea, Gross Gerhard, Lorenz Corinna, Menzel Henning, Windhagen Henning
Department of Orthopedic Surgery, Hannover Medical School, Germany.
J Appl Biomater Biomech. 2011 Jan-Apr;9(1):26-33. doi: 10.5301/JABB.2011.7731.
In modern orthopedics aseptic loosening caused by the formation of micro-wear particles remains a problem for endoprosthetic joint replacements as revision surgery is necessary with corresponding costs and exertions by patients. This study is devoted to the question of how the osseous ingrowth of implants can be supported. It was investigated whether the developed copolymer, p-VBP-co-GMA, coated on the surface of the implants, supports bone healing. In addition, it was analyzed whether covalent linkage of Bone Morphogenetic Protein 2 (BMP-2) to the copolymer layer enhances bone formation.
Eight adult New Zealand White Rabbits were implanted with four different foils (control, copolymer, copolymer + BMP-2, control + BMP-2) each. The histomorphometric analysis of all samples was made 28 days after implantation.
The copolymer had a positive effect on bone remodeling compared to the control group. We observed that the copolymer group had a significantly increased bone volume per tissue volume ratio and bone density to the control group. In contrast, this in-vivo study showed that the immobilization of BMP-2 onto the copolymer layer did not enhance bone healing. The bone volume per tissue volume ratio was decreased as well as the bone density compared to control + BMP-2 group.
The analysis showed that the bone remodeling process in the copolymer + BMP-2-group is in an early phase comparable to the control group. These results suggest that the coating with the developed copolymer has major potential for medical use as it enhances bone mass around the implant.
在现代骨科学中，由微磨损颗粒形成导致的无菌性松动仍然是人工关节置换的一个问题，因为需要进行翻修手术，这会给患者带来相应的费用和负担。本研究致力于探讨如何促进植入物的骨长入。研究了涂覆在植入物表面的新型共聚物p-VBP-co-GMA是否能促进骨愈合。此外，还分析了骨形态发生蛋白2（BMP-2）与共聚物层的共价连接是否能增强骨形成。
对8只成年新西兰白兔分别植入四种不同的箔片（对照组、共聚物组、共聚物+BMP-2组、对照组+BMP-2组）。植入28天后对所有样本进行组织形态计量学分析。
与对照组相比，共聚物对骨重塑有积极作用。我们观察到，与对照组相比，共聚物组的骨体积与组织体积比和骨密度显著增加。相比之下，这项体内研究表明，将BMP-2固定在共聚物层上并不能增强骨愈合。与对照组+BMP-2组相比，骨体积与组织体积比以及骨密度均降低。
分析表明，共聚物+BMP-2组的骨重塑过程处于与对照组相当的早期阶段。这些结果表明，用所开发的共聚物涂层具有很大的医学应用潜力，因为它能增加植入物周围的骨量。
