Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic.
Electrophoresis. 2011 Jun;32(13):1619-22. doi: 10.1002/elps.201000634. Epub 2011 May 24.
In this study, biotin-conjugated glutathione was synthesized using peptide bonding of the biotin carboxy group and amino group of the γ-glutamic acid to prepare an alternative coating for CdTe quantum dots (QDs). This type of coating combines the functionality of the biotin with the fluorescent properties of the QDs to create a specific, high-affinity fluorescent probe able to react with avidin, streptavidin and/or neutravidin. Biotin-functionalized glutathione-coated CdTe QDs were prepared by a simple one-step method using Na₂ TeO₃ and CdCl₂. Obtained QDs were separated from the excess of the biotin-conjugated glutathione by CE employing 300 mM borate buffer with pH 7.8 as a background electrolyte. The detection of sample components was performed by the photometric detection at 214 nm and LIF employing Ar⁺ ion laser (488 nm).
在这项研究中，使用生物素羧基和γ-谷氨酸氨基的肽键合成了生物素化谷胱甘肽，以制备 CdTe 量子点 (QD) 的替代涂层。这种涂层将生物素的功能与 QD 的荧光特性结合在一起，创建了一种具有特异性、高亲和力的荧光探针，能够与亲和素、链霉亲和素和/或中性亲和素反应。通过使用 Na₂TeO₃ 和 CdCl₂ 的简单一步法制备了生物素化谷胱甘肽涂层的 CdTe QD。通过 CE 用 300 mM 硼酸盐缓冲液（pH 值为 7.8）作为背景电解质，从过量的生物素化谷胱甘肽中分离出获得的 QD。通过在 214nm 处的光度检测和使用 Ar⁺离子激光（488nm）的 LIF 进行样品组分的检测。
