Rahman Md Musfiqur, Choi Jeong-Heui, Abd El-Aty A M, Park Jong Hyouk, Park Ji-Yeon, Im Geon-Jae, Shim Jae-Han
Natural Products Chemistry Laboratory, Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Science, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea.
Biomed Chromatogr. 2012 Feb;26(2):172-7. doi: 10.1002/bmc.1643. Epub 2011 May 24.
A simple analytical method was developed for the determination of chlorfenapyr residues in leeks grown under greenhouse conditions. Residues were extracted by salting out, analyzed by gas chromatography with microelectron-capture detection, and confirmed via gas chromatography-mass spectrometry. The calibration curves were found to be linear with correlation coefficients (r(2) ) in excess of 0.998. The limits of detection and quantification were 0.0015 and 0.005 mg kg(-1) , respectively. For validation purposes, recovery studies were carried out at low and high levels. Yield recovery rates were 87.27-89.64% with a relative standard deviation <6%. A maximum of 0.32 mg kg(-1) of chlorfenapyr residue was detected in leek sample sprayed three times at 7 day intervals until 7 days prior to harvest. The results of this study suggest that chlorfenapyr is acceptable for application in/on leeks under the recommended dosage regimen.
建立了一种简单的分析方法，用于测定温室条件下种植的韭菜中溴虫腈残留量。残留量通过盐析法提取，采用微电子捕获检测气相色谱法进行分析，并通过气相色谱-质谱联用进行确证。校准曲线呈线性，相关系数（r(2)）超过0.998。检测限和定量限分别为0.0015和0.005 mg kg(-1)。为进行验证，在低水平和高水平下开展了回收率研究。产量回收率为87.27 - 89.64%，相对标准偏差<6%。在收获前7天每隔7天喷施3次的韭菜样品中，检测到溴虫腈残留量最高为0.32 mg kg(-1)。本研究结果表明，按照推荐剂量方案在韭菜上施用溴虫腈是可接受的。
