小檗胺对佛波酯刺激的人多形核白细胞中活性氧自由基的清除作用。

Scavenging effect of berbamine on active oxygen radicals in phorbol ester-stimulated human polymorphonuclear leukocytes.

作者信息

Ju H S, Li X J, Zhao B L, Han Z W, Xin W J

机构信息

Institute of Biophysics, Academia Sinica, Beijing, China.

出版信息

Biochem Pharmacol. 1990 Jun 1;39(11):1673-8. doi: 10.1016/0006-2952(90)90110-7.

DOI:10.1016/0006-2952(90)90110-7

PMID:2160816

Abstract

The scavenging effect of berbamine (Ber) on active oxygen radicals was studied, using a spin-trapping technique and a chemiluminescence (CL) method in phorbol myristate acetate (PMA) stimulated polymorphonuclear leukocytes (PMN) and in four cell-free superoxide (O2-.) or hydroxyl radical (OH.) generating systems. Ber (0.1 to 0.3 mM) effectively reduced active oxygen radicals in PMN stimulated with PMA, but had no obvious effect on oxygen consumption during the respiratory burst of PMN, measured with spin probe oxymetry. Ber (0.3 mM) prominently inhibited the CL response of PMA-stimulated PMN. The agent remarkably quenched O2-. in xanthine/xanthine oxidase and irradiation riboflavin systems and OH. in the Fenton reaction. Its scavenging action on O2-. was stronger than that of Vitamin E in the xanthine/xanthine oxidase system but the same as Vitamin E in the riboflavin system, and its action on OH. was similar to that of Vitamin E.

摘要

利用自旋捕集技术和化学发光（CL）方法，在佛波酯（PMA）刺激的多形核白细胞（PMN）以及四种无细胞超氧化物（O2-.）或羟基自由基（OH.）产生系统中，研究了小檗胺（Ber）对活性氧自由基的清除作用。Ber（0.1至0.3 mM）可有效降低PMA刺激的PMN中的活性氧自由基，但用自旋探针测氧法测量时，对PMN呼吸爆发期间的氧消耗无明显影响。Ber（0.3 mM）显著抑制PMA刺激的PMN的CL反应。该试剂能显著淬灭黄嘌呤/黄嘌呤氧化酶和照射核黄素系统中的O2-.以及芬顿反应中的OH.。在黄嘌呤/黄嘌呤氧化酶系统中，其对O2-.的清除作用强于维生素E，但在核黄素系统中与维生素E相同，且其对OH.的作用与维生素E相似。