Zhao B L, Li X J, He R G, Cheng S J, Xin W J
Institute of Biophysics, Academia Sinica, Beijing, China.
Cell Biophys. 1989 Apr;14(2):175-85. doi: 10.1007/BF02797132.
With the use of the spin trapping methods, the scavenging effects of the extracts of green tea and other natural foods are studied. In stimulated polymorphonuclear leukocytes (PMN) system, water extract fraction 6 (F6) from green tea and green tea polyphenols (GTP) have the strongest scavenging effect on the active oxygen radicals, much stronger than vitamin C (Vc) and vitamin E (VE). Rosemary antioxidants (RA) and Curcumin (Cur) have weaker scavenging effects than Vc, but stronger than VE. In Fenton Reaction, Cur has the strongest scavenging effect (69%) on hydroxyl radicals. In irradiation, riboflavin system F6(74%) and GTP(72%) have very strong scavenging effects that are weaker than Vc, but much stronger than VE (23%). With the use of spin probe oxymetry, the oxygen consumption in respiratory burst of stimulated PMN were measured when the antioxidants existed in these systems. The results demonstrated that these antioxidants did not affect the respiratory burst of human polymorphonuclear leukocytes stimulated with PMA.
利用自旋捕集法,研究了绿茶提取物和其他天然食品的清除作用。在刺激的多形核白细胞(PMN)系统中,绿茶的水提取物组分6(F6)和绿茶多酚(GTP)对活性氧自由基具有最强的清除作用,比维生素C(Vc)和维生素E(VE)强得多。迷迭香抗氧化剂(RA)和姜黄素(Cur)的清除作用比Vc弱,但比VE强。在芬顿反应中,Cur对羟基自由基的清除作用最强(69%)。在辐射、核黄素系统中,F6(74%)和GTP(72%)具有非常强的清除作用,比Vc弱,但比VE(23%)强得多。利用自旋探针测氧法,在这些系统中存在抗氧化剂时,测量了刺激的PMN呼吸爆发中的耗氧量。结果表明,这些抗氧化剂不影响用佛波酯刺激的人多形核白细胞的呼吸爆发。