Department of Environmental Studies, Graduate School of Environmental Studies, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
Environ Sci Technol. 2011 Jun 15;45(12):5339-45. doi: 10.1021/es104199y. Epub 2011 May 24.
A dechlorinating consortium (designated as TES-1 culture) able to convert trichloroethene (TCE) to ethene was established from TCE-contaminated groundwater. This culture had the ability of complete dechlorination of TCE within about one month. From the clone library analysis of 16S rRNA gene, this culture was mainly composed of fermentation bacteria, such as Clostridium spp., and Desulfitobacterium spp. known as facultative dechlorinator. PCR using specific primers for Dehalococcoides spp. and the dehalogenase genes confirmed that the culture contained the Dehalococcoides spp. 16S rRNA gene and three dehalogenase genes, tceA, vcrA and bvcA. Dechlorination experiments using cis-dichloroethene (cis-DCE) at concentrations of 37-146 μM, revealed that the gene copy numbers of tceA, vcrA, and bvcA increased up to 10⁷ copy/mL, indicating that Dehalococcoides spp. containing these three dehalogenase genes were involved in cis-DCE dechlorination. However, in the culture to which 292 μM of cis-DCE was added, only the tceA gene and the Dehalococcoides spp. 16S rRNA gene increased up to 10⁷ copy/mL. The culture containing 292 μM of cis-DCE also exhibited about one tenth slower ethene production rate compared to the other cultures.
从受三氯乙烯 (TCE) 污染的地下水中建立了一个能够将三氯乙烯 (TCE) 转化为乙烯的脱氯共生体 (命名为 TES-1 培养物)。该培养物具有在大约一个月内完成 TCE 完全脱氯的能力。从 16S rRNA 基因的克隆文库分析来看，该培养物主要由发酵细菌组成，如梭菌属和脱硫菌属，它们被认为是兼性脱氯菌。使用针对 Dehalococcoides spp. 和脱卤酶基因的特异性引物进行 PCR 证实，该培养物含有 Dehalococcoides spp. 16S rRNA 基因和三个脱卤酶基因，tceA、vcrA 和 bvcA。使用浓度为 37-146 μM 的顺式-二氯乙烯 (cis-DCE) 进行脱氯实验表明，tceA、vcrA 和 bvcA 的基因拷贝数增加到 10⁷ 拷贝/mL，表明含有这三个脱卤酶基因的 Dehalococcoides spp. 参与了 cis-DCE 脱氯。然而，在添加了 292 μM 的 cis-DCE 的培养物中，只有 tceA 基因和 Dehalococcoides spp. 16S rRNA 基因增加到 10⁷ 拷贝/mL。与其他培养物相比，含有 292 μM 的 cis-DCE 的培养物的乙烯生成速率也慢了约十分之一。
