Behavioral and Metabolic Research Unit, School of Human Kinetics, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
Appl Physiol Nutr Metab. 2011 Apr;36(2):191-200. doi: 10.1139/h10-106.
Acute hyperinsulinemia reduces total and high molecular weight (HMW) adiponectin levels in humans. Whether an increase in insulin sensitivity (IS) is accompanied by a greater suppressive effect of hyperinsulinemia on adiponectin levels is unknown, however. To clarify the inhibitory role of insulin on adiponectin, total and HMW adiponectin levels were measured during acute hyperinsulinemia before and after an improvement in insulin sensitivity in response to weight loss. Forty-six overweight and obese postmenopausal women were randomized to either 6-month caloric restriction (CR) alone (n = 22), or CR with resistance training (CR+RT, n = 24). IS (hyperinsulinemic-euglycemic clamp) was assessed before and after weight loss. Total and HMW adiponectin levels were measured by ELISA at baseline, 90, 160, and 180 min of each clamp. Relative mean body weight loss was -8.0% ± 4.4% for both groups (CR: -7.7% ± 3.8%; CR+RT: -8.2% ± 5.0%). IS increased significantly, by 18.4% ± 25.3% (CR: 19.3% ± 29.7%; CR+RT: 17.7% ± 21.0%). Before each intervention, total and HMW adiponectin levels in both groups significantly decreased in response to hyperinsulinemia (total: -8.4% ± 19.4%; HMW: -3.2% ± 13.2%). Despite the improvement in IS seen after each intervention, a similar pattern of reduction to that before weight loss was observed in total and HMW adiponectin levels during hyperinsulinemia. These results establish that total and HMW adiponectin levels decline during a hyperinsulinemic-euglycemic clamp. Also, the insulin-sensitizing effect of weight loss via caloric restriction alone or with resistance training does not amplify the reduction in adiponectin levels observed during hyperinsulinemia in healthy postmenopausal women.
急性高胰岛素血症可降低人体总 adiponectin 和高分子量 (HMW) adiponectin 水平。然而，胰岛素敏感性 (IS) 增加是否伴随着高胰岛素血症对 adiponectin 水平的抑制作用增强尚不清楚。为了阐明胰岛素对 adiponectin 的抑制作用，在体重减轻引起的胰岛素敏感性改善前后，于急性高胰岛素血症期间测量了总 adiponectin 和 HMW adiponectin 水平。将 46 名超重和肥胖绝经后妇女随机分为单纯热量限制组（CR，n = 22）或热量限制联合阻力训练组（CR+RT，n = 24）。在体重减轻前后评估 IS（高胰岛素-正葡萄糖钳夹）。在基线、钳夹 90、160 和 180 分钟时，通过 ELISA 测量总 adiponectin 和 HMW adiponectin 水平。两组的相对平均体重减轻率分别为-8.0% ± 4.4%（CR：-7.7% ± 3.8%；CR+RT：-8.2% ± 5.0%）。IS 显著增加，增加了 18.4% ± 25.3%（CR：19.3% ± 29.7%；CR+RT：17.7% ± 21.0%）。在每个干预之前，两组的总 adiponectin 和 HMW adiponectin 水平在高胰岛素血症下均显著降低（总：-8.4% ± 19.4%；HMW：-3.2% ± 13.2%）。尽管在每个干预后 IS 都有所改善，但在高胰岛素血症期间，总 adiponectin 和 HMW adiponectin 水平仍呈现出与减肥前相似的降低模式。这些结果表明，在高胰岛素-正葡萄糖钳夹期间，总 adiponectin 和 HMW adiponectin 水平下降。此外，通过单纯热量限制或联合阻力训练减肥的胰岛素敏感性增强作用并不能放大健康绝经后妇女高胰岛素血症期间 adiponectin 水平的降低。
