Ebinger Katalin, Weller Harold N, Kiplinger Jeffrey, Lefebvre Paul
Bristol-Myers Squibb Company, Wallingford, CT, USA.
J Lab Autom. 2011 Jun;16(3):241-9. doi: 10.1016/j.jala.2010.03.005. Epub 2010 May 15.
Preparative HPLC-MS is often the method of choice for purification of small amounts (<100mg) of diverse new molecules, such as compound libraries for drug discovery. The method is robust, well proven, and widely applicable. In contrast, preparative supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) has seen only slow acceptance for the same application--despite some potential scientific and economic advantages. One of the reasons for slow adoption of SFC-MS is the lack of well-proven, robust, and commercially available instrumentation. In early 2009, TharSFC (a Waters Company, Pittsburgh, PA) introduced a new fully integrated system for preparative SFC-MS: The SFC-MS Prep-100. We report herein an objective evaluation of the SFC-MS Prep-100, including tests for pump and autosampler performance, sample recovery, sample carryover, fraction triggering, detector/fraction collector synchronization, and overall robustness. Our results suggest that the SFC-MS Prep-100 represents a significant advance over previous generation instrumentation.
制备型高效液相色谱-质谱联用（Preparative HPLC-MS）通常是纯化少量（<100mg）各种新分子的首选方法，例如用于药物发现的化合物库。该方法性能可靠，久经考验，且应用广泛。相比之下，制备型超临界流体色谱与质谱联用（SFC-MS）在相同应用中的接受度增长缓慢——尽管它具有一些潜在的科学和经济优势。SFC-MS采用率低的原因之一是缺乏经过充分验证、性能可靠且商业化的仪器。2009年初，TharSFC（位于宾夕法尼亚州匹兹堡的沃特世公司）推出了一款用于制备型SFC-MS的全新全集成系统：SFC-MS Prep-100。我们在此报告对SFC-MS Prep-100的客观评估结果，包括对泵和自动进样器性能、样品回收率、样品残留、馏分触发、检测器/馏分收集器同步以及整体稳定性的测试。我们的结果表明SFC-MS Prep-100相较于上一代仪器有显著进步。
